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体外表皮屏障形成的定量蛋白质基因组分析

Quantitative proteogenomic profiling of epidermal barrier formation in vitro.

作者信息

Winget Jason M, Watts Julian D, Hoopmann Michael R, DiColandrea Teresa, Robinson Michael K, Huggins Tom, Bascom Charles C, Isfort Robert J, Moritz Robert L

机构信息

Institute for Systems Biology, 401 Terry Ave N., Seattle, WA 98109, USA.

The Procter & Gamble Company, Mason Business Center, Cincinnati, OH 45040, USA.

出版信息

J Dermatol Sci. 2015 Jun;78(3):173-80. doi: 10.1016/j.jdermsci.2015.02.013. Epub 2015 Mar 14.

DOI:10.1016/j.jdermsci.2015.02.013
PMID:25862149
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4424144/
Abstract

BACKGROUND

The barrier function of the epidermis is integral to personal well-being, and defects in the skin barrier are associated with several widespread diseases. Currently there is a limited understanding of system-level proteomic changes during epidermal stratification and barrier establishment.

OBJECTIVE

Here we report the quantitative proteogenomic profile of an in vitro reconstituted epidermis at three time points of development in order to characterize protein changes during stratification.

METHODS

The proteome was measured using data-dependent "shotgun" mass spectrometry and quantified with statistically validated label-free proteomic methods for 20 replicates at each of three time points during the course of epidermal development.

RESULTS

Over 3600 proteins were identified in the reconstituted epidermis, with more than 1200 of these changing in abundance over the time course. We also collected and discuss matched transcriptomic data for the three time points, allowing alignment of this new dataset with previously published characterization of the reconstituted epidermis system.

CONCLUSION

These results represent the most comprehensive epidermal-specific proteome to date, and therefore reveal several aspects of barrier formation and skin composition. The limited correlation between transcript and protein abundance underscores the importance of proteomic analysis in developing a full understanding of epidermal maturation.

摘要

背景

表皮的屏障功能对个人健康至关重要，皮肤屏障缺陷与多种常见疾病相关。目前，对于表皮分层和屏障建立过程中系统水平的蛋白质组学变化了解有限。

目的

在此，我们报告体外重构表皮在三个发育时间点的定量蛋白质基因组图谱，以表征分层过程中的蛋白质变化。

方法

在表皮发育过程中的三个时间点，每个时间点进行20次重复实验，使用数据依赖的“鸟枪法”质谱测定蛋白质组，并采用经统计学验证的无标记蛋白质组学方法进行定量。

结果

在重构表皮中鉴定出3600多种蛋白质，其中1200多种在整个时间过程中丰度发生变化。我们还收集并讨论了这三个时间点匹配的转录组数据，使这个新数据集能够与先前发表的重构表皮系统特征进行比对。

结论

这些结果代表了迄今为止最全面的表皮特异性蛋白质组，因此揭示了屏障形成和皮肤组成的几个方面。转录本和蛋白质丰度之间的有限相关性强调了蛋白质组学分析在全面理解表皮成熟过程中的重要性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6999/4424144/bc68d072cc0b/nihms672109f7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6999/4424144/05b06f44ddc6/nihms672109f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6999/4424144/0d115253fb14/nihms672109f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6999/4424144/d7194498ffba/nihms672109f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6999/4424144/78c71b387685/nihms672109f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6999/4424144/5a7dafbbe6cd/nihms672109f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6999/4424144/e0af31bacbe7/nihms672109f6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6999/4424144/bc68d072cc0b/nihms672109f7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6999/4424144/05b06f44ddc6/nihms672109f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6999/4424144/0d115253fb14/nihms672109f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6999/4424144/d7194498ffba/nihms672109f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6999/4424144/78c71b387685/nihms672109f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6999/4424144/5a7dafbbe6cd/nihms672109f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6999/4424144/e0af31bacbe7/nihms672109f6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6999/4424144/bc68d072cc0b/nihms672109f7.jpg

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本文引用的文献

1
Transcriptional profiling of epidermal barrier formation in vitro.体外表皮屏障形成的转录谱分析。
J Dermatol Sci. 2014 Mar;73(3):187-97. doi: 10.1016/j.jdermsci.2013.11.004. Epub 2013 Nov 11.
2
Protein biomarkers in vernix with potential to predict the development of atopic eczema in early childhood.胎脂中的蛋白质生物标志物，有可能预测儿童早期特应性皮炎的发生。
Allergy. 2014 Jan;69(1):104-12. doi: 10.1111/all.12308. Epub 2013 Nov 11.
3
Consistency of the proteome in primary human keratinocytes with respect to gender, age, and skin localization.原发性人角质形成细胞中蛋白质组在性别、年龄和皮肤定位方面的一致性。
Mol Cell Proteomics. 2013 Sep;12(9):2509-21. doi: 10.1074/mcp.M112.025478. Epub 2013 May 30.
4
Comet: an open-source MS/MS sequence database search tool.彗星：一个开源的 MS/MS 序列数据库搜索工具。
Proteomics. 2013 Jan;13(1):22-4. doi: 10.1002/pmic.201200439. Epub 2012 Dec 4.
5
A cross-platform toolkit for mass spectrometry and proteomics.一个用于质谱和蛋白质组学的跨平台工具包。
Nat Biotechnol. 2012 Oct;30(10):918-20. doi: 10.1038/nbt.2377.
6
iProphet: multi-level integrative analysis of shotgun proteomic data improves peptide and protein identification rates and error estimates.iProphet：高通量蛋白质组学数据的多层次综合分析可提高肽段和蛋白质的鉴定率和错误评估。
Mol Cell Proteomics. 2011 Dec;10(12):M111.007690. doi: 10.1074/mcp.M111.007690. Epub 2011 Aug 29.
7
The generating function of CID, ETD, and CID/ETD pairs of tandem mass spectra: applications to database search.串联质谱的 CID、ETD 和 CID/ETD 对的生成函数：在数据库搜索中的应用。
Mol Cell Proteomics. 2010 Dec;9(12):2840-52. doi: 10.1074/mcp.M110.003731. Epub 2010 Sep 9.
8
Intercellular junction assembly, dynamics, and homeostasis.细胞间连接的组装、动态变化和内稳性。
Cold Spring Harb Perspect Biol. 2010 Feb;2(2):a000125. doi: 10.1101/cshperspect.a000125.
9
Molecular basis of the core structure of tight junctions.紧密连接核心结构的分子基础。
Cold Spring Harb Perspect Biol. 2010 Jan;2(1):a002907. doi: 10.1101/cshperspect.a002907.
10
Physiology and function of the tight junction.紧密连接的生理学和功能。
Cold Spring Harb Perspect Biol. 2009 Aug;1(2):a002584. doi: 10.1101/cshperspect.a002584.