Zhao Jun, Zhu Tianhui, He Liumei, Shen Xiaoli, Wang Yanjun, Deng Zhihui
Shenzhen Eye Hospital Affiliated to Jinan University, Shenzhen, Guangdong 518040, P.R.China.
Zhonghua Yi Xue Yi Chuan Xue Za Zhi. 2015 Apr;32(2):254-8. doi: 10.3760/cma.j.issn.1003-9406.2015.02.023.
To assess the association of HLA-DPA1 and -DPB1 polymorphisms with Posner-Schlossman syndrome (PSS) in southern Chinese Han population.
A total of 100 randomly selected PSS patients of southern Chinese Han origin were served as the experimental group, while 128 unrelated healthy blood donors of the same origin were served as the control group. All samples were subjected to sequencing-based typing (SBT) for exon 2 of HLA-DPA1 and -DPB1 loci in both directions. HLA genotype was assigned using an Assign 3.5 HLA SBT software. The allele frequencies and haplotype frequencies of HLA-DPA1 and -DPB1 of the two groups were compared. x² test, P value and odds ratio (OR) value were calculated.
Six HLA-DPA1 alleles in the experimental group and 4 HLA-DPA1 alleles in the healthy control group were identified. The allelic frequency for HLA-DPA102:01 in the experimental group was significantly lower than the control group (4.50% vs. 12.109%; x²=8.124, P=0.004). Sixteen HLA-DPB1 alleles were identified in both the experimental and control groups. The allelic frequencies for HLA-DPB114:01 and - DPB117:01 in the experimental group were significantly lower than those of the control group ( DPB114:01: 1.00% vs. 4.688%, x²=5.130, P=0.024; DPB117:01: 0% vs. 2.344%, x²=3.897, P=0.048). The DPA1-DPB1 haplotypes for the experimental and control groups were 23 and 25, respectively. The haplotype frequencies for both DPA102:01- DPB114:01 and DPA102:01- DPB1*17:01 were significantly lower than those of the control group.
DPA102:01- DPB114:01 and DPA102:01- DPB117:01 haplotypes may provide considerable protection effect against PSS in the southern Chinese Han population.
评估中国南方汉族人群中HLA - DPA1和 - DPB1基因多态性与波斯纳 - 施洛斯曼综合征（PSS）的相关性。
随机选取100例中国南方汉族PSS患者作为实验组，128例同种族无关健康献血者作为对照组。所有样本均对HLA - DPA1和 - DPB1基因座的第2外显子进行双向测序分型（SBT）。使用Assign 3.5 HLA SBT软件确定HLA基因型。比较两组HLA - DPA1和 - DPB1的等位基因频率和单倍型频率。计算卡方检验、P值和比值比（OR）值。
实验组鉴定出6种HLA - DPA1等位基因，健康对照组鉴定出4种HLA - DPA1等位基因。实验组中HLA - DPA102:01的等位基因频率显著低于对照组（4.50%对12.109%；χ² = 8.124，P = 0.004）。实验组和对照组均鉴定出16种HLA - DPB1等位基因。实验组中HLA - DPB114:01和 - DPB117:01的等位基因频率显著低于对照组（DPB114:01：1.00%对4.688%，χ² = 5.130，P = 0.024；DPB117:01：0%对2.344%，χ² = 3.897，P = 0.048）。实验组和对照组的DPA1 - DPB1单倍型分别为23种和25种。DPA102:01 - DPB114:01和DPA102:01 - DPB1*17:01的单倍型频率均显著低于对照组。
DPA102:01 - DPB114:01和DPA102:01 - DPB117:01单倍型可能对中国南方汉族人群的PSS提供显著的保护作用。
