Neucel Specialty Cellulose Ltd, PO Box 2000, 300 Marine Drive, Port Alice, BC V0N 2N0, Canada.
Lorena School of Engineering, University of São Paulo Estrada Municipal do Campinho s/n, CP 116, 12602-810 Lorena, SP, Brazil.
Bioresour Technol. 2015;187:288-298. doi: 10.1016/j.biortech.2015.03.084. Epub 2015 Mar 30.
The influence of chip size and moisture content on the combined sugar recovery after steam pretreatment of lodgepole pine and subsequent enzymatic hydrolysis of the cellulosic component were investigated using response surface methodology. Chip size had little influence on sugar recovery after both steam pretreatment and enzymatic hydrolysis. In contrast, the moisture of the chips greatly influenced the relative severity of steam pretreatment and, as a result, the combined sugar recovery from the hemicellulosic and cellulosic fractions. Irrespective of chip size and the pretreatment temperature, time, and SO2 loading that were used, the relative severity of pretreatment was highest at a moisture of 30-40w/w%. However, the predictive model indicated that an elevated moisture content of roughly 50w/w% (about the moisture content of a standard softwood mill chip) would result in the highest, combined sugar recovery (80%) over the widest range of steam pretreatment conditions.
采用响应面法研究了木屑尺寸和水分含量对云杉蒸汽预处理后复合糖回收率的影响，以及随后对纤维素成分进行酶水解的影响。木屑尺寸对蒸汽预处理后和酶水解后的糖回收率影响不大。相比之下，木屑的水分极大地影响了蒸汽预处理的相对剧烈程度，从而影响了半纤维素和纤维素部分的复合糖回收率。无论木屑尺寸以及所用的预处理温度、时间和 SO2 负荷如何，在水分含量为 30-40w/w%时，预处理的相对剧烈程度最高。然而，预测模型表明，水分含量升高至约 50w/w%（略高于标准软木片厂木屑的水分含量），在最广泛的蒸汽预处理条件下，可获得最高的复合糖回收率（80%）。
