Rodriguez Annette R, Plascencia-Villa Germán, Witt Colleen M, Yu Jieh-Juen, José-Yacamán Miguel, Chambers James P, Perry George, Guentzel M Neal, Arulanandam Bernard P
RCMI Biophotonics Core, University of Texas at San Antonio, One UTSA Circle, San Antonio, TX 78249, United States.
RCMI Nanotechnology and Human Health Core, Department of Physics, University of Texas at San Antonio, One UTSA Circle, San Antonio, TX 78249, United States.
Cell Immunol. 2015 Jun;295(2):83-91. doi: 10.1016/j.cellimm.2015.03.010. Epub 2015 Apr 1.
The human pathogen Chlamydia pneumoniae has been implicated in chronic inflammatory diseases including type 2 diabetes. Therefore, we designed a study to evaluate pancreatic beta cells and mast cells during chlamydial infection. Our study revealed that C. pneumoniae infected mast cells significantly (p<0.005) decreased beta cell ATP and insulin production, in contrast to uninfected mast cells co-cultured with beta cells. Infected mast cells exhibited pyknotic nuclei and active caspase-3 and caspase-1 expression. Additionally, ex vivo analyses of tissues collected from C. pneumoniae infected mice showed increased interleukin-1β production in splenocytes and pancreatic tissues as was observed with in vitro mast cell-beta cell co-cultures during C. pneumoniae infection. Notably, infected mast cells promoted beta cell destruction. Our findings reveal the negative effect of C. pneumoniae on mast cells, and the consequential impact on pancreatic beta cell function and viability.
人类病原体肺炎衣原体与包括2型糖尿病在内的慢性炎症性疾病有关。因此，我们设计了一项研究来评估衣原体感染期间的胰腺β细胞和肥大细胞。我们的研究表明，与与β细胞共培养的未感染肥大细胞相比，肺炎衣原体感染的肥大细胞显著（p<0.005）降低了β细胞的三磷酸腺苷（ATP）和胰岛素生成。受感染的肥大细胞表现出核固缩以及活性半胱天冬酶-3和半胱天冬酶-1的表达。此外，对从肺炎衣原体感染小鼠收集的组织进行的体外分析显示，脾细胞和胰腺组织中白细胞介素-1β的生成增加，这与肺炎衣原体感染期间体外肥大细胞与β细胞共培养时观察到的情况一致。值得注意的是，受感染的肥大细胞促进了β细胞的破坏。我们的研究结果揭示了肺炎衣原体对肥大细胞产生的负面影响，以及对胰腺β细胞功能和活力的相应影响。
