School of Natural Resources and Geography, Bangor University, Bangor, Gwynedd LL57 2UW, UK.
BioComposites Centre, Bangor University, Bangor, Gwynedd LL57 2UW, UK.
Bioresour Technol. 2015;189:81-86. doi: 10.1016/j.biortech.2015.03.056. Epub 2015 Mar 18.
Pressure refining, a pulp making process to separate fibres of lignocellulosic materials, deposits lignin granules on the surface of the fibres that could enable increased access to lignin degrading enzymes. Three different white rot fungi were grown on pressure refined (at 6 bar and 8 bar) and milled Miscanthus. Growth after 28 days showed highest biomass losses on milled Miscanthus compared to pressure refined Miscanthus. Ceriporiopsis subvermispora caused a significantly higher proportion of lignin removal when grown on 6 bar pressure refined Miscanthus compared to growth on 8 bar pressure refined Miscanthus and milled Miscanthus. RM22b followed a similar trend but Phlebiopsis gigantea SPLog6 did not. Conversely, C. subvermispora growing on pressure refined Miscanthus revealed that the proportion of cellulose increased. These results show that two of the three white rot fungi used in this study showed higher delignification on pressure refined Miscanthus than milled Miscanthus.
压力精炼是一种将木质纤维素材料的纤维分离的制浆工艺，会在纤维表面沉积木质素颗粒，从而增加木质素降解酶的可及性。三种不同的白腐真菌在压力精炼（6 巴和 8 巴）和研磨芒草上生长。28 天后的生长情况表明，与压力精炼芒草相比，研磨芒草的生物量损失最高。与生长在 8 巴压力精炼芒草和研磨芒草上相比，Ceriporiopsis subvermispora 在 6 巴压力精炼芒草上生长时，木质素去除率显著更高。RM22b 呈现出类似的趋势，但 Phlebiopsis gigantea SPLog6 则不然。相反，在压力精炼芒草上生长的 C. subvermispora 表明纤维素的比例增加了。这些结果表明，在本研究中使用的三种白腐真菌中有两种在压力精炼芒草上的脱木质素程度高于研磨芒草。
