高血压血瘀证患者血清孵育的内皮细胞中微小RNA的差异表达
Differential expression of microRNA in endothelial cells incubated with serum of hypertension patients with blood-stasis syndrome.
作者信息
Wang Ming-ming, Fang Mei-xia, Chen Li-guo, Wang Hua-qiang, Liu Hong-jie, Tang Hai-lan
机构信息
Faculty of Traditional Chinese Medicine, Medical School of Jinan University, Guangzhou, 510632, China.
出版信息
Chin J Integr Med. 2015 Nov;21(11):817-22. doi: 10.1007/s11655-015-2053-1. Epub 2015 Apr 11.
OBJECTIVE
To screen out blood-stasis syndrome (BSS)-associated microRNA and therefore determine the possible target for treating hypertension.
METHODS
A high-energy sequencing method and digital gene expression sequencing theory were adopted to sequence microRNA (miRNA) and messenger RNA (mRNA), and to determine differential expression in human umbilical vein endothelial cells incubated with serum samples from hypertension patients with or without BSS, and healthy controls. The results were confirmed using gene prediction software.
RESULTS
A total of 13 miRNAs and 11 mRNAs showed statistical difference both in the BSS/normal groups and BSS/non-BSS groups, respectively. Four pairs of target mRNA/miRNA were identified: FRMD4A/hsa-miR-34a, MAP3K14/hsa-miR-34a, PER1/hsa-miR-34a, and FGF2/hsa-miR-132.
CONCLUSION
Four mRNA/miRNA pairs mentioned above seem to be involved in pathogenesis and maintenance of hypertension with BSS.
目的
筛选出与血瘀证相关的微小RNA，从而确定治疗高血压的可能靶点。
方法
采用高通量测序方法和数字基因表达测序理论对微小RNA（miRNA）和信使核糖核酸（mRNA）进行测序，以确定在分别用伴有或不伴有血瘀证的高血压患者血清样本以及健康对照血清样本孵育的人脐静脉内皮细胞中的差异表达。结果用基因预测软件进行验证。
结果
分别在血瘀证/正常组和血瘀证/非血瘀证组中，共有13种miRNA和11种mRNA表现出统计学差异。鉴定出四对靶mRNA/miRNA：FRMD4A/hsa-miR-34a、MAP3K14/hsa-miR-34a、PER1/hsa-miR-34a和FGF2/hsa-miR-132。
结论
上述四对mRNA/miRNA似乎参与了伴有血瘀证的高血压的发病机制和维持过程。
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