子痫前期患者中抗炎介质脂氧素A4及炎症反应转录因子核因子-κB的表达

Expression of anti-inflammatory mediator lipoxin A4 and inflammation responsive transcriptive factors NF-kappa B in patients with preeclampsia.

作者信息

Huang L L, Su S, Awale R, Zhang X Y, Zhong L L, Tang H

出版信息

Clin Exp Obstet Gynecol. 2014;41(5):561-6.

PMID:25864260

Abstract

OBJECTIVE

To evaluate the role of lipoxin A4 (LXA4), NF-kappaB p65 in preeclampsia.

MATERIALS AND METHODS

LXA4 in blood serum and the lipoxin A4 receptor (ALX-R), NF-kappaB p65 mRNA, protein expressions in placenta-specific tissues were obtained from patients with preeclampsia and normal pregnancy.

RESULTS

Levels of lipoxin A4 in women with mild preeclampsia was significantly high (p < 0.05). There was no significant statistical difference between normal pregnancy and severe preeclampsia (p > 0.05). The mRNA expression of ALX-R was significantly low in women with preeclampsia than in control group (p < 0.01) and mRNA expression of NF- kappaB p65 was significantly high in preeclampsia (p < 0.01). The immunohistochemical staining of NF-kappaB p65 protein was stronger in severe preeclampsia group whereas staining of ALX-R in placental tissue was weaker than in control group (p < 0.01). ALX-R mRNA was negatively correlated with NF-kappaB (p < 0.0001), but there was no correlation between LXA4 and ALX-R mRNA.

CONCLUSION

There was an excessive maternal inflammatory response in preeclampsia. LXA4, ALX-R, and NF-kappaB p65 may be involved in the disease process ofpreeclampsia.

摘要

目的

评估脂氧素A4（LXA4）、核因子κB p65在子痫前期中的作用。

材料与方法

收集子痫前期患者及正常妊娠患者血清中的LXA4、胎盘组织中脂氧素A4受体（ALX-R）、核因子κB p65的mRNA及蛋白表达情况。

结果

轻度子痫前期患者血清中脂氧素A4水平显著升高（p<0.05）。正常妊娠与重度子痫前期之间无显著统计学差异（p>0.05）。子痫前期患者ALX-R的mRNA表达显著低于对照组（p<0.01），而核因子κB p65的mRNA表达在子痫前期显著升高（p<0.01）。重度子痫前期组核因子κB p65蛋白的免疫组化染色更强，而胎盘组织中ALX-R的染色弱于对照组（p<0.01）。ALX-R mRNA与核因子κB呈负相关（p<0.0001），但LXA4与ALX-R mRNA之间无相关性。