Fukuhara Shigetomo, Fukui Hajime, Wakayama Yuki, Ando Koji, Nakajima Hiroyuki, Mochizuki Naoki
Department of Cell Biology, National Cerebral and Cardiovascular Center Research Institute, 5-7-1 Fujishirodai, Suita, Osaka, 565-8565, Japan.
JST-CREST, National Cerebral and Cardiovascular Center, 5-7-1, Suita, Osaka, 565-8565, Japan.
Dev Growth Differ. 2015 May;57(4):333-40. doi: 10.1111/dgd.12210. Epub 2015 Apr 10.
Development requires cell proliferation, migration, differentiation, apoptosis, and many kinds of cell responses. Cells prepare intracellular conditions to respond to extracellular cues from neighboring cells. We have studied the development of the cardiovascular system (CVS) by visualizing morphology and signaling simultaneously using zebrafish, which express probes for both. Endodermal sheet is required for the bilateral cardiac precursor cell (CPC) migration toward the midline. Endothelial cells (ECs) proliferate specifically in the certain regions of blood vessels. Bone morphogenetic proteins (BMP) induce the remodeling of the caudal vein plexus (CVP) to form the caudal vein (CV). Our findings point to the pre-existing neighboring cells as the cells exhibiting certain responses during the development of CVS. In this review, we introduce recent results of our research on angiogenesis and cardiogenesis by spotlighting the mechanism by which ECs and CPCs are regulated by the cells next to themselves. In addition, we discuss the unanswered questions that should be clarified in the future in the field of CVS development.
发育需要细胞增殖、迁移、分化、凋亡以及多种细胞反应。细胞会准备细胞内环境以响应来自相邻细胞的细胞外信号。我们利用斑马鱼同时可视化形态和信号传导，对心血管系统（CVS）的发育进行了研究，斑马鱼能同时表达这两种探针。双侧心脏前体细胞（CPC）向中线迁移需要内胚层片层。内皮细胞（EC）在血管的特定区域特异性增殖。骨形态发生蛋白（BMP）诱导尾静脉丛（CVP）重塑形成尾静脉（CV）。我们的研究结果表明，在CVS发育过程中，预先存在的相邻细胞是表现出特定反应的细胞。在这篇综述中，我们通过聚焦EC和CPC如何被其相邻细胞调节的机制，介绍我们关于血管生成和心脏发生的最新研究结果。此外，我们还讨论了CVS发育领域未来应阐明的未解决问题。
