Savela K, Hemminki K, Hewer A, Phillips D H, Putman K L, Randerath K
Institute of Occupational Health, Helsinki, Finland.
Mutat Res. 1989 Dec;224(4):485-92. doi: 10.1016/0165-1218(89)90074-8.
Analysis by nuclease P1-enhanced 32P-postlabelling assay of DNA isolated from the white blood cells of 53 iron foundry workers was carried out independently in 3 laboratories, and the presence of aromatic DNA adducts was detected. The mean adduct levels in foundry workers varied from 9.2 +/- 23 (laboratory 3) and 12 +/- 10 (laboratory 2) to 26 +/- 43 (laboratory 1) and for the controls from 1.7 +/- 0.7 (laboratory 3) to 3.1 +/- 1.7 (laboratory 1) adducts per 10(8) nucleotides. No effect of smoking was observed in the present study. Each laboratory observed large interindividual variations of adduct levels. Good correlations were found between the results of the 32P-postlabelling assays carried out in the 3 laboratories; the correlation coefficients between laboratories 1 and 2, 1 and 3, and 2 and 3 were 0.61, 0.62, and 0.45, respectively, all being statistically highly significant (p less than 0.01). This interlaboratory comparison of the 32P-postlabelling method indicates the reproducibility of the method and its applicability in occupational exposure monitoring.
对53名铸铁厂工人白细胞中分离出的DNA进行核酸酶P1增强的32P后标记分析，该分析在3个实验室独立开展，并检测到芳香族DNA加合物的存在。铸铁厂工人的平均加合物水平每10(8)个核苷酸从9.2±23（实验室3）和12±10（实验室2）到26±43（实验室1）不等，对照组则从每10(8)个核苷酸1.7±0.7（实验室3）到3.1±1.7（实验室1）不等。本研究未观察到吸烟的影响。每个实验室都观察到加合物水平存在较大的个体间差异。3个实验室进行的32P后标记分析结果之间存在良好的相关性；实验室1和2、1和3、2和3之间的相关系数分别为0.61、0.62和0.45，均具有高度统计学显著性（p<0.01）。32P后标记法的这种实验室间比较表明了该方法的可重复性及其在职业暴露监测中的适用性。
