Ho Jin-Nyoung, Kim Ok-Kyung, Nam Da-Eun, Jun Woojin, Lee Jeongmin
Department of Medical Nutrition, Kyung Hee University.
J Nutr Sci Vitaminol (Tokyo). 2014;60(6):429-35. doi: 10.3177/jnsv.60.429.
This study investigated the PKA-dependent inhibitory effect of pycnogenol (Pyc) on lipolysis using ob/ob mice and primary mouse adipocytes. Supplementation of Pyc at 30 mg/kg significantly reduced body weight gain and visceral fat mass. The serum and hepatic triglyceride (TG) and total cholesterol (TC) levels were reduced by Pyc supplementation, and high density lipoprotein (HDL)-cholesterol level significantly increased. In addition, hormone sensitive lipase (HSL) and adipose triglyceride lipase (ATGL) mRNA levels increased with Pyc supplementation in adipose tissue of ob/ob mice. The treatment of primary cultured adipocytes with Pyc at 100 μg/mL significantly increased glycerol release, cAMP level by reduction of phosphodiestersae-3B (PDE3B), and HSL levels, but decreased protein levels of perilipin A and fatty acid synthetase (FAS). The PKA inhibitor (H89) clearly blocked the cellular levels of perilipin A and HSL, suggesting that Pyc promotes lipolysis of adipocytes through activation of cAMP-dependent PKA, resulting in induction of HSL and reduction of perilipin A. Therefore, this study may elucidate the possible mechanism of Pyc, which is a candidate for weight loss through stimulation of lipolysis.
本研究使用ob/ob小鼠和原代小鼠脂肪细胞，调查了碧萝芷（Pyc）对脂解作用的蛋白激酶A（PKA）依赖性抑制作用。以30mg/kg的剂量补充碧萝芷可显著降低体重增加和内脏脂肪量。补充碧萝芷可降低血清和肝脏甘油三酯（TG）及总胆固醇（TC）水平，高密度脂蛋白（HDL）胆固醇水平显著升高。此外，在ob/ob小鼠的脂肪组织中，补充碧萝芷会使激素敏感性脂肪酶（HSL）和脂肪甘油三酯脂肪酶（ATGL）的mRNA水平升高。用100μg/mL的碧萝芷处理原代培养的脂肪细胞，可显著增加甘油释放、通过降低磷酸二酯酶-3B（PDE3B）提高cAMP水平以及提高HSL水平，但降低了围脂滴蛋白A和脂肪酸合成酶（FAS）的蛋白水平。PKA抑制剂（H89）明显阻断了围脂滴蛋白A和HSL的细胞水平，表明碧萝芷通过激活cAMP依赖性PKA促进脂肪细胞的脂解作用，从而导致HSL的诱导和围脂滴蛋白A的减少。因此，本研究可能阐明了碧萝芷的潜在机制，碧萝芷是通过刺激脂解作用实现减肥的候选物质。
