Wen Z H, Zeng Y X, Ji Z J, Yang C D
State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou, China.
State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou, China
Genet Mol Res. 2015 Mar 6;14(1):1636-49. doi: 10.4238/2015.March.6.10.
Rice sheath blight (ShB), which is caused by Rhizoctonia solani, has become the most serious rice disease in China. Yangdao 4, a cultivar with partial resistance to ShB, was crossed with Lemont, a susceptible cultivar, to develop mapping populations that were used to analyze quantitative trait loci (QTL) that confer resistance to ShB. QTL analysis were performed in 3 environments (E1-E3) using 2 F2 and 1 F2:3 populations, respectively. Three traits were recorded to evaluate ShB resistance, including disease rating (DR), lesion height (LH), and percentage of lesion height (PLH). Based on field evaluation of ShB resistance and the 2 genetic maps constructed, we identified a total of 8 QTLs for DR (4 in E1, 4 in E2, and 3 in E3), 6 QTLs for LH (1 in E1, 3 in E2, and 2 in E3), and 7 QTLs for PLH (1 in E1, 4 in E2, and 2 in E3). Sixteen of the ShB-QTLs co-localized as 6 clusters on chromosomes 3, 7, 11, and 12. Four of the 6 clusters contained ShB-QTLs that were detected in 2 environments, while the other 2 clusters with ShB-QTLs were detected in 1 environment. Three ShB-QTLs (qSBD-3-2, qSBL-3-1, and qSBPL-3-1) were delimited to a 581-kb region flanked by markers D333B and D334 on chromosome 3. The resistance alleles of Yangdao 4 at the qSBD-3-2 locus decreased DR by 0.68 and 0.79 in E2 and E3, respectively.
由立枯丝核菌引起的水稻纹枯病已成为中国最严重的水稻病害。对水稻纹枯病具有部分抗性的品种扬稻4号与感病品种Lemont杂交，构建定位群体，用于分析赋予水稻纹枯病抗性的数量性状位点（QTL）。分别使用2个F2群体和1个F2:3群体在3种环境（E1 - E3）中进行QTL分析。记录了3个用于评估水稻纹枯病抗性的性状，包括病情等级（DR）、病斑高度（LH）和病斑高度百分比（PLH）。基于对水稻纹枯病抗性的田间评估以及构建的2个遗传图谱，我们共鉴定出8个控制DR的QTL（E1中4个，E2中4个，E3中3个），6个控制LH的QTL（E1中1个，E2中3个，E3中2个），以及7个控制PLH的QTL（E1中1个，E2中4个，E3中2个）。16个水稻纹枯病QTL共定位于第3、7、11和12号染色体上的6个簇中。6个簇中的4个包含在2种环境中检测到的水稻纹枯病QTL，而另外2个含有水稻纹枯病QTL的簇仅在1种环境中检测到。3个水稻纹枯病QTL（qSBD - 3 - 2、qSBL - 3 - 1和qSBPL - 3 - 1）被定位到第3号染色体上标记D333B和D334两侧的一个581 kb区域内。在qSBD - 3 - 2位点，扬稻4号的抗性等位基因在E2和E3中分别使病情等级降低了0.68和0.79。
