猪生长过程中不同组织线粒体DNA拷贝数的定量变化
Quantitative changes in mitochondrial DNA copy number in various tissues of pigs during growth.
作者信息
Xie Y M, Jin L, Chen X J, He M N, Wang Y, Liu R, Li M Z, Li X W
机构信息
Institute of Animal Genetics and Breeding, College of Animal Science and Technology, Sichuan Agricultural University, Ya'an, Sichuan, China.
Institute of Animal Genetics and Breeding, College of Animal Science and Technology, Sichuan Agricultural University, Ya'an, Sichuan, China
出版信息
Genet Mol Res. 2015 Mar 6;14(1):1662-70. doi: 10.4238/2015.March.6.12.
Mitochondrial DNA (mtDNA) content is dependent on the energy requirements of tissues. To date, no comprehensive study has been conducted to examine mtDNA copy number variations in pigs. In the current study, quantitative polymerase chain reaction was performed to quantify the mtDNA copy number in 15 pig tissue types at 5 growth stages from embryo to adult. Observable differences in mtDNA content were detected in the tissues, including a 6-fold greater mtDNA content in the heart compared with the lung of 180-day-old samples. mtDNA content in the heart, longissimus dorsi muscle, psoas major muscle, kidney, brain, ovary, and subcutaneous adipose increased with growth. Expression of the replicative mitochondrial helicase (TWINKLE), which regulates mtDNA turnover, was significantly associated with changes in mtDNA copy numbers across tissues during growth (r = 0.33, P = 0.01). We demonstrated that the expression levels of mitochondrial genes were positively correlated with mtDNA copy number.
线粒体DNA（mtDNA）含量取决于组织的能量需求。迄今为止，尚未进行全面研究来检测猪的mtDNA拷贝数变异。在本研究中，进行了定量聚合酶链反应以量化从胚胎到成年的5个生长阶段的15种猪组织类型中的mtDNA拷贝数。在这些组织中检测到了mtDNA含量的明显差异，包括180日龄样本中，心脏的mtDNA含量比肺高6倍。心脏、背最长肌、腰大肌、肾脏、大脑、卵巢和皮下脂肪中的mtDNA含量随生长而增加。调节mtDNA周转的复制性线粒体解旋酶（TWINKLE）的表达与生长过程中各组织mtDNA拷贝数的变化显著相关（r = 0.33，P = 0.01）。我们证明线粒体基因的表达水平与mtDNA拷贝数呈正相关。
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