A-FABP基因的过表达促进转基因小鼠的肌间脂肪沉积。
Overexpression of the A-FABP gene facilitates intermuscular fat deposition in transgenic mice.
作者信息
Liu Z W, Fan H L, Liu X F, Ding X B, Wang T, Sui G N, Li G P, Guo H
机构信息
College of Animal Science and Animal Medicine, TianJin Agricultural University, TianJin, China.
Key Laboratory of National Education Ministry for Mammalian Reproductive Biology and Biotechnology, Inner Mongolia University, Hohhot, China
出版信息
Genet Mol Res. 2015 Mar 31;14(1):2742-9. doi: 10.4238/2015.March.31.4.
Adipocyte fatty acid-binding protein (A-FABP), the most abundant FABP in adipocytes, controls fatty acid uptake, transport, and metabolism in fat cells. We constructed a transgenic mice model that overexpressed the cattle A-FABP gene to investigate the relationship between A-FABP expression and intermuscular fat deposition. There was no significant difference in body weight and serum biochemical indexes between transgenic and wild-type mice. Further, there were no significant differences in intermuscular triglyceride content and A-FABP expression levels over three generations of transgenic mice. However, abdominal adipose rate, A-FABP protein content, and intermuscular triglyceride levels of transgenic mice were significantly higher than those of wild-type mice. In addition, triglycerides were remarkably higher in the skeletal muscle but lower in the myocardium of transgenic mice. Thus, overexpression of cattle A-FABP gene promoted fat deposition in the skeletal muscle of transgenic mice.
脂肪细胞脂肪酸结合蛋白（A-FABP）是脂肪细胞中含量最丰富的脂肪酸结合蛋白，它控制着脂肪细胞中脂肪酸的摄取、运输和代谢。我们构建了一个过表达牛A-FABP基因的转基因小鼠模型，以研究A-FABP表达与肌间脂肪沉积之间的关系。转基因小鼠和野生型小鼠在体重和血清生化指标上没有显著差异。此外，三代转基因小鼠的肌间甘油三酯含量和A-FABP表达水平也没有显著差异。然而，转基因小鼠的腹部脂肪率、A-FABP蛋白含量和肌间甘油三酯水平显著高于野生型小鼠。此外，转基因小鼠骨骼肌中的甘油三酯显著升高，而心肌中的甘油三酯则降低。因此，牛A-FABP基因的过表达促进了转基因小鼠骨骼肌中的脂肪沉积。
Zotero 插件