Sharkey K A, Sobrino J A, Cervero F, Varro A, Dockray G J
Department of Physiology, Medical School, University of Bristol, U.K.
Neuroscience. 1989;32(1):169-79. doi: 10.1016/0306-4522(89)90116-4.
The origin of calcitonin gene-related peptide in the thoracic spinal cord of the rat was investigated by radioimmunoassay and immunohistochemistry. In transverse sections from normal animals there was a dense staining of calcitonin gene-related peptide-immunoreactivity in laminae I, II and V of the dorsal horn. In parasagittal sections this was found to consist of rostrocaudally orientated fibres in laminae I and II and longitudinal bundles of fibres interspersed with a plexus of immunoreactivity in lamina V. After sectioning the thoracic spinal nerves there was a significant reduction in immunoreactivity in the dorsal horn of the spinal cord which was seen as a marked reduction of staining in lamina II and in the bundles of fibres in lamina V. Section of the splanchnic nerve slightly reduced staining in lamina I and virtually abolished the plexuses of immunoreactivity in lamina V. However, measurement of calcitonin gene-related peptide in samples from coeliac-ganglionized rats revealed an increase in immunoreactivity in regions of the spinal cord containing lamina V. These results provide evidence of a visceral and somatic afferent origin of calcitonin gene-related peptide in the thoracic spinal cord of the rat.
通过放射免疫测定法和免疫组织化学方法，对大鼠胸段脊髓中降钙素基因相关肽的起源进行了研究。在正常动物的横切面上，背角的I、II和V层有密集的降钙素基因相关肽免疫反应性染色。在矢状旁切面上，发现这由I和II层中头向尾方向排列的纤维以及V层中纤维纵向束与免疫反应性丛相互交织组成。切断胸段脊神经后，脊髓背角的免疫反应性显著降低，表现为II层染色明显减少以及V层纤维束染色减少。切断内脏神经使I层染色略有减少，并几乎消除了V层的免疫反应性丛。然而，对来自腹腔神经节化大鼠样本中降钙素基因相关肽的测量显示，脊髓中含V层区域的免疫反应性增加。这些结果为大鼠胸段脊髓中降钙素基因相关肽的内脏和躯体传入起源提供了证据。
