Liaudat A C, Rodríguez N, Chen S, Romanini M C, Vivas A, Rolando A, Gauna H, Mayer N
Department of Molecular Biology, School of Exact, Physical-Chemical and Natural Sciences, National University of Río Cuarto , Río Cuarto , Argentina.
Biotech Histochem. 2015;90(6):432-8. doi: 10.3109/10520295.2015.1019926. Epub 2015 Apr 13.
Stress in pregnant rats caused by chronic immobilization alters the pattern of secretion of corticosterone and modifies the hypothalamic-pituitary-adrenal axis (HPA) of the fetus. Early postnatal handling, however, may reverse the effects of increased secretion of corticosterone. We investigated the effects of prenatal stress and postnatal handling on the activity of the HPA axis of male offspring of stressed female rats. Male 90-day-old rats from four groups were investigated: prenatally stressed animals without postnatal handling, prenatally stressed animals with postnatal handling, unstressed control animals with postnatal handling, and unstressed control animals without postnatal handling. After sacrifice, the adrenal glands were weighed to determine the adrenal-somatic index. Apoptosis was evaluated by TUNEL assay and active caspase-3 expression. We found that the adrenal gland cortex:medulla ratio increased in animals with prenatal stress and that eventually the stress caused apoptosis. Handling newborns to simulate maternal activity ameliorated some of the negative effects of prenatal stress.
慢性固定导致的孕鼠应激会改变皮质酮的分泌模式，并改变胎儿的下丘脑-垂体-肾上腺轴（HPA）。然而，出生后早期的处理可能会逆转皮质酮分泌增加的影响。我们研究了产前应激和产后处理对应激雌性大鼠雄性后代HPA轴活性的影响。对来自四组的90日龄雄性大鼠进行了研究：产前应激且未进行产后处理的动物、产前应激且进行产后处理的动物、未应激且进行产后处理的对照动物以及未应激且未进行产后处理的对照动物。处死后，称量肾上腺重量以确定肾上腺-躯体指数。通过TUNEL检测和活性半胱天冬酶-3表达评估细胞凋亡。我们发现，产前应激动物的肾上腺皮质：髓质比值增加，最终应激导致细胞凋亡。处理新生动物以模拟母体活动可改善产前应激的一些负面影响。
