Pena Edwin A, Ridley Lauren M, Murphy Wyatt R, Sowa John R, Bentivegna Carolyn S
Department of Biological Sciences, Seton Hall University, South Orange, New Jersey, USA.
Department of Chemistry and Biochemistry, Seton Hall University, South Orange, New Jersey, USA.
Environ Toxicol Chem. 2015 Sep;34(9):1946-58. doi: 10.1002/etc.3015. Epub 2015 Jun 30.
Raw menhaden fish oil was developed for biomonitoring polycyclic aromatic hydrocarbons (PAHs) using fluorescence spectroscopy. Menhaden (Genus Brevoortia) were collected in 2010 and/or 2011 from Delaware Bay, New Jersey, USA; James River, Virginia, USA; Vermillion Bay, Louisiana, USA (VBLA); and Barataria Bay, Louisiana, USA (BBLA). Barataria Bay, Louisiana received heavy oiling from the Deepwater Horizon oil spill. Method development included determining optimal wavelengths for PAH detection, fish oil matrix interferences, and influence of solvent concentration on extraction. Results showed that some fish oils contained high molecular weight PAH-like compounds in addition to other fluorescent compounds such as albumin and vitamin A and vitamin E. None of these naturally occurring compounds interfered with detection of high molecular weight PAHs. However, data suggested that the lipid component of fish oil was altering fluorescence spectra by supporting the formation of PAH excimers. For example, the most intense excitation wavelength for hydroxypyrene shifted from Ex285/Em430 to Ex340/Em430. Comparison of Deepwater Horizon crude oil and fish oil spectra indicated that some fish oils contained crude oil-like PAHs. Using wavelengths of Ex360/Em430, fish oil concentrations were calculated as 3.92 μg/g, 0.61 μg/g, and 0.14 μg/g for a Delaware Bay sample, BBLA 2011, and VBLA 2011, respectively. Overall, these results supported using menhaden fish oil to track PAH exposures spatially and temporally.
粗制鲱鱼油被开发用于利用荧光光谱法进行多环芳烃（PAHs）的生物监测。2010年和/或2011年从美国新泽西州特拉华湾、美国弗吉尼亚州詹姆斯河、美国路易斯安那州 Vermillion 湾（VBLA）以及美国路易斯安那州巴拉塔里亚湾（BBLA）采集了鲱鱼（Brevoortia 属）。路易斯安那州的巴拉塔里亚湾遭受了深水地平线漏油事件的严重油污污染。方法开发包括确定 PAH 检测的最佳波长、鱼油基质干扰以及溶剂浓度对提取的影响。结果表明，一些鱼油除了含有白蛋白、维生素A和维生素E等其他荧光化合物外，还含有高分子量的类PAH化合物。这些天然存在的化合物均未干扰高分子量PAHs的检测。然而，数据表明鱼油的脂质成分通过支持PAH激基缔合物的形成改变了荧光光谱。例如，羟基芘的最强激发波长从Ex285/Em430 移至 Ex340/Em430。深水地平线原油和鱼油光谱的比较表明，一些鱼油含有类似原油的PAHs。使用Ex360/Em430波长，特拉华湾样品、2011年的BBLA和2011年的VBLA的鱼油浓度分别计算为3.92μg/g、0.61μg/g和0.14μg/g。总体而言，这些结果支持使用鲱鱼油在空间和时间上追踪PAH暴露情况。
