Chen Huiqiang, Cui Wei, Hu Haijuan, Liu Jing
Department of Cardiology, The Second Hospital of Hebei Medical University, Shijiazhuang; Hebei-China.
Anatol J Cardiol. 2015 Nov;15(11):893-6. doi: 10.5152/akd.2014.5817. Epub 2014 Dec 31.
Culturing aortic valve interstitial cells is a useful way to investigate the physiology and pathology of the aortic valve at the cellular level. The culture methods of the cells have been established in many species. However, the previous methods need some improvements.
We evaluated various techniques with regard to the isolation of Sprague-Dawley (SD) rat aortic valve interstitial cells and established suitable conditions about the culture and passage of the primary cells. The specimens from the aortic valve were processed by tissue explant methods before seeding them onto the dishes.
The cells obtained emerged from the explants after 2 to 3 days and stained positive for α-SMA and vimentin protein. Moreover, transmission electron microscopy images showed that the cells had abundant mitochondria, prominent rough endoplasmic reticulum, and plentiful myofilaments.
In the present study, we provided reliable and efficient methods for the isolation and culture of rat aortic valve interstitial cells that could serve for in vitro studies on aortic valve physiology and pathophysiology.
培养主动脉瓣间质细胞是在细胞水平研究主动脉瓣生理和病理的一种有用方法。细胞培养方法已在许多物种中建立。然而，以前的方法需要一些改进。
我们评估了多种分离Sprague-Dawley（SD）大鼠主动脉瓣间质细胞的技术，并确定了原代细胞培养和传代的合适条件。将主动脉瓣标本用组织块法处理后接种到培养皿上。
接种后2至3天，外植体上出现细胞，α-SMA和波形蛋白染色呈阳性。此外，透射电镜图像显示细胞内线粒体丰富，粗面内质网突出，肌丝丰富。
在本研究中，我们提供了可靠且高效的大鼠主动脉瓣间质细胞分离和培养方法，可用于主动脉瓣生理和病理生理学的体外研究。
