Marsala J, Sulla I, Santa M, Marsala M, Mechírová E, Jalc P
Institute of Neurobiology, Centre of Physiological Sciences, Slovak Academy of Sciences, Kosice, Czechoslovakia.
Neurosci Lett. 1989 Nov 20;106(1-2):83-8. doi: 10.1016/0304-3940(89)90206-1.
Mapping the canine lumbosacral spinal cord neurons damaged by ischemia-reperfusion after high thoracic aorta ligation was performed using the Nauta degenerating method. Highly Nauta-positive perikarya of the long ascending projection systems in the 4th to 6th dorsal layer, interneurons in the 7th layer and motoneurons in the 8th and 9th layers in L3-S3 segments subjected to 30 min of ischemia and 30 min of reperfusion were localized and their laminar distribution was specified. Spastic paraplegia fully developed 2 days postoperatively after 30 min of aortic ligation is neurohistopathologically characterized by occurrence of enlarged Nauta-positive boutons with prevailing localization in the 4th to 8th layer of the gray matter.
采用Nauta变性法对高位胸主动脉结扎后缺血再灌注损伤的犬腰骶部脊髓神经元进行定位。对L3-S3节段经历30分钟缺血和30分钟再灌注的第4至6背层长升支投射系统的高Nauta阳性核周体、第7层中间神经元以及第8和9层运动神经元进行定位，并明确其层状分布。主动脉结扎30分钟后，术后2天痉挛性截瘫完全发展，其神经组织病理学特征为出现增大的Nauta阳性终扣，主要位于灰质的第4至8层。
