Li Xiangfeng, Lu Jiang, Lu Jing, Hu Xiaoxiao, Huang Zhiyong
a College of Life Science, University of Chinese Academy of Sciences , Beijing , China and.
b BGI-Shenzhen , Shenzhen , China.
Mitochondrial DNA A DNA Mapp Seq Anal. 2016 Nov;27(6):4213-4214. doi: 10.3109/19401736.2015.1022745. Epub 2015 Apr 14.
The American crow, Corvus brachyrhynchos (Passeriformes, Corvidae), is a large passerine bird species closely related to the raven. Herein, we first published the complete mitochondrial genome of American crows. The mitogenome was 16,917 bp long, and composed of 13 protein-coding genes, 22 tRNA genes, two rRNA genes, and one putative control region. Most protein-coding genes started with a traditional ATG codon except for COI, which initiated with an infrequent start codon GTG instead, and terminated with the mitochondria stop codon (TAA/AGG/AGA) or a single T base. The mitogenome structural organization is identical to that of the other corvus species and related genera. The overall GC content is 44.25% which is lower than AT. Using the 12 protein-coding genes of Corvus brachyrhynchos in this study, together with 10 other closely species, we constructed the species phylogenetic tree to verify the accuracy and utility of new determined mitogenome sequences. We expect that using the full mitogenome to address taxonomic issues and study the related evolution events. Moreover, this is the first report of bird mitogenomes after 48 avian species genome project achievements released in December 2014.
美洲鸦（Corvus brachyrhynchos，雀形目，鸦科）是一种大型雀形目鸟类，与渡鸦亲缘关系密切。在此，我们首次公布了美洲鸦的完整线粒体基因组。该线粒体基因组长度为16,917 bp，由13个蛋白质编码基因、22个tRNA基因、2个rRNA基因和1个假定的控制区组成。除COI基因外，大多数蛋白质编码基因以传统的ATG密码子起始，而COI基因则以罕见的起始密码子GTG起始，并以线粒体终止密码子（TAA/AGG/AGA）或单个T碱基终止。线粒体基因组的结构组织与其他鸦属物种及相关属相同。总体GC含量为44.25%，低于AT含量。在本研究中，我们利用美洲鸦的12个蛋白质编码基因，以及其他10个近缘物种，构建了物种系统发育树，以验证新测定的线粒体基因组序列的准确性和实用性。我们期望利用完整的线粒体基因组来解决分类学问题并研究相关的进化事件。此外，这是在2014年12月发布48种鸟类基因组计划成果之后，关于鸟类线粒体基因组的首次报道。
