小鼠精子中组蛋白去乙酰化酶/乙酰转移酶的表达水平与随后的囊胚发育之间存在联系吗？

Is There a Link Between Expression Levels of Histone Deacetylase/Acetyltransferase in Mouse Sperm and Subsequent Blastocyst Development?

作者信息

Kim Jayeon, Kim Ji-Hee, Jee Byung-Chul, Suh Chang-Suk, Kim Seok-Hyun

机构信息

Department of Obstetrics and Gynecology, Cha Gangnam Hospital, Cha University, Seoul, South Korea Department of Obstetrics and Gynecology, Seoul National University College of Medicine, Seoul, South Korea.

Department of Obstetrics and Gynecology, Seoul National University Bundang Hospital, Bundang, South Korea.

出版信息

Reprod Sci. 2015 Nov;22(11):1387-92. doi: 10.1177/1933719115580997. Epub 2015 Apr 12.

DOI:10.1177/1933719115580997

PMID:25868580

Abstract

Histone acetylation has been known to be significant in spermatogenesis. Histone acetylation is regulated by the act of histone deacetylases (HDACs) and histone acetyltransferases (HATs). We investigated the link between expression levels of HDACs and HATs in mouse sperm and subsequent blastocyst formation rate. In the univariate analysis, expression levels of HDAC1 and HAT were generally not associated with the blastocyst formation rate. When divided by the mature oocyte number category, a significant positive association was observed between the expression levels of HDAC1 and the blastocyst-forming rate in the highest (> 75th) percentile group (a group with ≥34 mature oocytes). In conclusion, expression of sperm HDAC1 could be considered as a possible predictor of embryo development in mice with high ovarian response.

摘要

组蛋白乙酰化在精子发生过程中具有重要意义，这一点已为人所知。组蛋白乙酰化受组蛋白去乙酰化酶（HDACs）和组蛋白乙酰转移酶（HATs）的调控。我们研究了小鼠精子中HDACs和HATs的表达水平与后续囊胚形成率之间的联系。在单因素分析中，HDAC1和HAT的表达水平通常与囊胚形成率无关。当按成熟卵母细胞数量类别划分时，在最高（>第75百分位数）百分位组（成熟卵母细胞≥34个的组）中，观察到HDAC1的表达水平与囊胚形成率之间存在显著正相关。总之，精子HDAC1的表达可被视为高卵巢反应小鼠胚胎发育的一个可能预测指标。