Erkoc Reha, Cikrikcioglu Mehmet Ali, Aintab Emre, Toprak Aybala Erek, Kilic Ulkan, Gok Ozlem, Cetin Ayse Irem Yasin, Zorlu Mehmet, Kiskac Muharrem, Cakirca Mustafa, Erkal Sena Nur, Isen Hayati Can, Karatoprak Cumali
a Division of Nephrology, Department of Internal Medicine, Faculty of Medicine , Bezmialem Vakif University , Istanbul , Turkey .
Ren Fail. 2015 Jun;37(5):866-70. doi: 10.3109/0886022X.2015.1034606. Epub 2015 Apr 14.
BACKGROUND - AIM: In animal experiments, growth arrest-specific 6 (Gas6) protein plays a key role in the development of mesangial cell and glomerular hypertrophy in the early phase of diabetic nephropathy, and diabetic nephropathy is prevented by warfarin-induced inhibition of GAS6 protein. It was shown that GAS6 intron 8 c.834 + 7G > A polymorphism is protective against type 2 diabetes mellitus, and AA genotype is associated with higher blood levels of GAS6 protein. Our aim is to investigate whether this polymorphism is a risk factor for diabetic nephropathy in type 2 diabetes mellitus.
Eighty-seven patients with diabetic nephropathy were compared with 66 non-diabetic controls in terms of GAS6 intron 8 c.834 + 7G > A polymorphism. Patients with history of stroke, ischemic heart disease were excluded. Each patient was examined by the ophthalmologist to determine diabetic retinopathy.
Frequency of GG, GA and AA genotypes are similar in diabetic nephropathy and control groups according to GAS6 intron 8 c.834 + 7G > A polymorphism (p = .837). Rate of diabetic retinopathy was 54.02%. In the subgroup analysis, GA genotype was significantly more frequent than GG genotype in patients with diabetic retinopathy when compared to without diabetic retinopathy (p = .010).
In our study, GAS6 intron 8 c.834 + 7G > A polymorphism was not associated with diabetic nephropathy in type 2 diabetes mellitus. However, heterozygous state of this polymorphism may be a risk factor for diabetic retinopathy in patients with diabetic nephropathy.
背景 - 目的：在动物实验中，生长停滞特异性蛋白6（Gas6）在糖尿病肾病早期的系膜细胞发育和肾小球肥大中起关键作用，华法林诱导的Gas6蛋白抑制可预防糖尿病肾病。研究表明，Gas6内含子8 c.834 + 7G>A多态性对2型糖尿病具有保护作用，AA基因型与较高的Gas6蛋白血水平相关。我们的目的是研究这种多态性是否是2型糖尿病患者发生糖尿病肾病的危险因素。
比较87例糖尿病肾病患者和66例非糖尿病对照者的Gas6内含子8 c.834 + 7G>A多态性。排除有中风、缺血性心脏病史的患者。每位患者均由眼科医生检查以确定糖尿病视网膜病变。
根据Gas6内含子8 c.834 + 7G>A多态性，糖尿病肾病组和对照组的GG、GA和AA基因型频率相似（p = 0.837）。糖尿病视网膜病变发生率为54.02%。在亚组分析中，与无糖尿病视网膜病变的患者相比，有糖尿病视网膜病变的患者中GA基因型显著多于GG基因型（p = 0.010）。
在我们的研究中，Gas6内含子8 c.834 + 7G>A多态性与2型糖尿病患者的糖尿病肾病无关。然而，这种多态性的杂合状态可能是糖尿病肾病患者发生糖尿病视网膜病变的危险因素。
