Krnajski Z, Geering S, Steadman S
Forensic Biology/DNA Laboratory, Sedgwick County Regional Forensic Science Center, 1109 North Minneapolis, 67214, Wichita, KS, USA.
Forensic Sci Med Pathol. 2007 Dec;3(4):264-9. doi: 10.1007/s12024-007-0034-1.
Advances in automation have been made for a number of processes conducted in the forensic DNA laboratory. However, because most robotic systems are designed for high-throughput laboratories batching large numbers of samples, smaller laboratories are left with a limited number of cost-effective options for employing automation. The Maxwell 16 Instrument and DNA IQ Reference Sample Kit marketed by Promega are designed for rapid, automated purification of DNA extracts from sample sets consisting of sixteen or fewer samples. Because the system is based on DNA capture by paramagnetic particles with maximum binding capacity, it is designed to generate extracts with yield consistency. The studies herein enabled evaluation of STR profile concordance, consistency of yield, and cross-contamination performance for the Maxwell 16 Instrument. Results indicate that the system performs suitably for streamlining the process of extracting known reference samples generally used for forensic DNA analysis and has many advantages in a small or moderate-sized laboratory environment.
法医DNA实验室中进行的许多流程在自动化方面都取得了进展。然而，由于大多数机器人系统是为处理大量样本的高通量实验室设计的，较小的实验室在采用自动化方面的经济高效选择有限。普洛麦格公司销售的麦克斯韦16仪器和DNA IQ参考样本试剂盒旨在从包含16个或更少样本的样本组中快速、自动纯化DNA提取物。由于该系统基于具有最大结合能力的顺磁颗粒捕获DNA，其设计目的是产生产量一致的提取物。本文的研究能够评估麦克斯韦16仪器的STR图谱一致性、产量一致性和交叉污染性能。结果表明，该系统在简化通常用于法医DNA分析的已知参考样本提取过程方面表现良好，并且在小型或中型实验室环境中有许多优势。
