Ondriska F, Bukovinova P, Votypka J, Nohynkova E, Boldis V
Bratisl Lek Listy. 2015;116(3):203-6. doi: 10.4149/bll_2015_040.
We present a case of imported leishmaniasis in a 31-year-old woman from Slovakia, who visited the countries of South America for three months in 2011. On 29 and 31 August 2011, she was probably infected with Leishmania parasites in the jungles of Ecuador. Approximately one week after returning to Slovakia, a small papules appeared on patient's left leg. Another wound was found after two weeks. Both ulcers were enlarging. We proved amastigote forms of Leishmania spp. only in repeated dermal scrapings from the edge of the ulcer by Giemsa staining after negative results from examination of a wound scrape and biopsy specimen. We identified the species Leishmania (Viannia) panamensis as a causative agent by using the polymerase chain reaction (PCR) method and subsequent sequencing of the ITS region. Closure of wounds and scab formation were observed after 20 days of treatment with sodium stibogluconate. In the control microscopic examination after the end of the treatment, parasites were not present, and the PCR confirmed the negative result (Fig. 2, Ref. 31).
我们报告一例来自斯洛伐克的31岁女性输入性利什曼病病例，该患者于2011年在南美洲国家旅行了三个月。2011年8月29日和31日，她可能在厄瓜多尔的丛林中感染了利什曼原虫。回到斯洛伐克大约一周后，患者左腿出现一个小丘疹。两周后发现另一个伤口。两个溃疡都在扩大。在伤口刮片和活检标本检查结果为阴性后，我们仅通过吉姆萨染色在溃疡边缘反复进行的皮肤刮片中证实了利什曼原虫的无鞭毛体形式。我们通过聚合酶链反应（PCR）方法及随后对ITS区域的测序，确定病原体为巴拿马利什曼原虫（Viannia亚属）。在用葡萄糖酸锑钠治疗20天后，观察到伤口闭合和结痂形成。治疗结束后的对照显微镜检查中未发现寄生虫，PCR也证实了阴性结果（图2，参考文献31）。
