Pan Feng, Chen Liang, Ding Fei, Zhang Jie, Gu Yu-Dong
Department of Hand Surgery, Wuxi Number 9 People's Hospital Affiliated to Soochow University, Wuxi, Jiangsu Province 214062, the People's Republic of China.
Department of Hand Surgery, Huashan Hospital and Institutes of Biomedical Sciences, Fudan University, Shanghai 200040, the People's Republic of China; Shanghai Key Laboratory of Peripheral Nerve and Microsurgery, Shanghai 200040, the People's Republic of China.
Gene. 2015 Jul 10;565(2):268-74. doi: 10.1016/j.gene.2015.04.012. Epub 2015 Apr 11.
Clinically in obstetric brachial plexus palsy (OBPP), irreversible atrophy of intrinsic musculature of the hand in denervation occurs much earlier than that of denervated arm muscles. With the aim of finding clues to explain this, the miRNA expression profile of denervated intrinsic musculature of the forepaw (IMF) and that of the denervated biceps were examined by microarray screening in the rat model simulating irreversible muscular atrophy caused by pan-plexus lesions of OBPP, and potential targets of specifically dysregulated miRNAs were predicted with use of bioinformatics analysis. It was found that denervated IMF and biceps had their own specifically dysregulated miRNAs, respectively, as compared with control. Analysis of Gene Ontology and of pathway showed that those specifically dysregulated miRNAs and their target genes might participate in self-regulation of neuromuscular junctions (NMJs). These outcomes suggest that self-regulative mechanism of NMJs may be different between denervated IMF and denervated biceps in the rat model simulating irreversible muscular atrophy of OBPP.
临床上，在产科臂丛神经麻痹（OBPP）中，去神经支配的手部固有肌肉组织的不可逆萎缩比去神经支配的手臂肌肉出现得更早。为了找到解释这一现象的线索，在模拟OBPP全丛病变导致的不可逆肌肉萎缩的大鼠模型中，通过微阵列筛选检查了去神经支配的前爪固有肌肉组织（IMF）和去神经支配的二头肌的miRNA表达谱，并利用生物信息学分析预测了特异性失调miRNA的潜在靶标。结果发现，与对照组相比，去神经支配的IMF和二头肌分别有各自特异性失调的miRNA。基因本体分析和通路分析表明，这些特异性失调的miRNA及其靶基因可能参与神经肌肉接头（NMJ）的自我调节。这些结果表明，在模拟OBPP不可逆肌肉萎缩的大鼠模型中，去神经支配的IMF和去神经支配的二头肌之间NMJ的自我调节机制可能不同。
