皮下移植后使用纤维蛋白凝胶加血小板裂解液对分离的卵巢窦前卵泡进行形态学研究。
Morphological study of isolated ovarian preantral follicles using fibrin gel plus platelet lysate after subcutaneous transplantation.
作者信息
Rajabzadeh Ali Reza, Eimani Hossein, Mohseni Koochesfahani Homa, Shahvardi Abdol-Hossein, Fathi Rouhollah
机构信息
Department of Embryology at Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran ; Department of Cell and Molecular Biology, Faculty of Biological Sciences, Kharazmi University, Tehran, Iran.
Department of Embryology at Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran ; Department of Anatomy, Faculty of Medicine, Baqiyatallah (a.s.) University of Medical Sciences, Tehran, Iran.
出版信息
Cell J. 2015 Spring;17(1):145-52. doi: 10.22074/cellj.2015.521. Epub 2015 Apr 8.
OBJECTIVE
Ovarian and follicle transplantation may preserve fertility in young cancer survivors. In this study, we have transplanted preantral follicles using fibrin gel as a carrier and fibrin gel supplemented with platelet lysate (PL) as a rich source of angiogenic and growth factors. The purpose of this study was to evaluate the role of fibrin gel and PL in follicle transplantation.
MATERIALS AND METHODS
In this experimental study, ovaries were taken from 14-day- old Naval Medical Research Institute (NMRI) mice. Preantral follicles were dissected from the ovaries and encapsulated into fibrin gel supplemented with 5, 10, 15 or 20% PL, then transplanted back into the same donor mice. Fibrin gels supplemented with PL that contained preantral follicles were placed in a subcutaneous pocket in the back of the neck of the recipient, donor mouse (the same mouse that follicles were collected). After 14 days the grafts were processed and embedded in paraffin blocks, then serially sectioned for histological evaluation. We counted the follicles and classified them according to stage (preantral or antral). Data were presented as mean ± standard error of mean (SEM) and analysed by analysis of variance (ANOVA) and the Kruskal-Wallistest.
RESULTS
The mean percentage of recovered follicles encapsulated and transplanted in each group were 33.30 ± 2.47 (fibrin gel), 31.96 ± 1.90 (fibrin gel+5% PL), 34.02 ± 2.44 (fibrin gel+10% PL), 48.31 ± 2.06 (fibrin gel+15% PL) and 17.60 ± 2.79 (fibrin gel+20% PL). There was a significant increase in the recovery rate of grafted follicles with fibrin gel+15% PL (48.31%; p<0.001). The percentage of preantral follicles showed no significant difference in all groups (p<0.05). The percentage of antral follicles showed a significant decrease in follicles grafted with fibrin gel+20% PL when compared to the other groups (11.77%; p<0.005) but no significant difference was observed in the other groups.
CONCLUSION
The use of PL in follicle transplantation can improve ovarian follicular survival rate.
目的
卵巢和卵泡移植可能会保留年轻癌症幸存者的生育能力。在本研究中，我们使用纤维蛋白凝胶作为载体以及补充了血小板裂解物（PL）的纤维蛋白凝胶（作为血管生成和生长因子的丰富来源）来移植窦前卵泡。本研究的目的是评估纤维蛋白凝胶和PL在卵泡移植中的作用。
材料与方法
在本实验研究中，从14日龄的海军医学研究所（NMRI）小鼠身上获取卵巢。从卵巢中解剖出窦前卵泡，并将其封装到补充有5%、10%、15%或20%PL的纤维蛋白凝胶中，然后再移植回同一供体小鼠体内。将含有窦前卵泡且补充了PL的纤维蛋白凝胶放置在受体供体小鼠（收集卵泡的同一小鼠）颈部后方的皮下囊袋中。14天后，对移植物进行处理并包埋在石蜡块中，然后进行连续切片以进行组织学评估。我们对卵泡进行计数并根据阶段（窦前或窦状）进行分类。数据以平均值±平均标准误差（SEM）表示，并通过方差分析（ANOVA）和Kruskal-Wallis检验进行分析。
结果
每组中封装并移植的回收卵泡的平均百分比分别为33.30±2.47（纤维蛋白凝胶）、31.96±1.90（纤维蛋白凝胶+5%PL）、34.02±2.44（纤维蛋白凝胶+10%PL）、48.31±2.06（纤维蛋白凝胶+15%PL）和17.60±2.79（纤维蛋白凝胶+20%PL）。纤维蛋白凝胶+15%PL组的移植卵泡回收率显著增加（48.31%；p<0.001）。所有组中窦前卵泡百分比无显著差异（p<0.05）。与其他组相比，纤维蛋白凝胶+20%PL移植的卵泡中窦状卵泡百分比显著降低（11.77%；p<0.005），但其他组未观察到显著差异。
结论
在卵泡移植中使用PL可提高卵巢卵泡存活率。
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