Bhat B, Sharma V D
Indian J Exp Biol. 2015 Mar;53(3):184-8.
Valeriana officinalis is an important medicinal herb commonly found in Kashmir valley. This study forms an important preliminary step for in-vitro micro propagation of V. officinalis from breaking the seed dormancy, inducing rapid seed germination and its subsequent micro propagation. We investigated the influence of pretreatment of V. officinalis seeds with reduced temperature and light on seed germination and in-vitro propagation. Culture of explants from cultivated seeds have demonstrated its potential for in vitro propagation and plantlet regeneration. Individual as well as combinations of treatments such as temperature and light availability influenced the germination of seeds variedly. Unchilled seeds of V. officinalis were given dip in GA3 (200 ppm) for 24, 48 and 120 h. Seeds treated with GA3 for 24 h and kept in darkness showed the best results, i.e. 48%. Seeds pretreated with GA3 for 120 h and incubated in dark showed 40% germination. Pre-chilling up to 72 h and kept in light showed maximum germination of 60% followed by 40% kept in darkness. Pre-chilling for 48 h resulted in 40 and 25% seed germination in light and darkness, respectively. GA3 pre-treatment for 72 h and 24 h pre chilling were most effective in inducing seed germination. Maximum shoot response was obtained on MS enriched with BAP (1 mg/L) + IAA (0.1 mg/L) combinations using shoot tips as explants. Multiple shoot regeneration from shoot apices was recorded on BAP (1 mg/L) and BAP (1 mg/L) + IAA (0.1 mg/L).
缬草是克什米尔山谷常见的一种重要草药。本研究是缬草体外微繁殖的重要初步步骤，包括打破种子休眠、诱导种子快速萌发及其后续的微繁殖。我们研究了低温和光照预处理缬草种子对种子萌发和体外繁殖的影响。培养栽培种子的外植体已证明其体外繁殖和植株再生的潜力。温度和光照等单独处理以及处理组合对种子萌发有不同影响。缬草未冷藏的种子分别在GA3（200 ppm）中浸泡24、48和120小时。用GA3处理24小时并置于黑暗中的种子效果最佳，发芽率为48%。用GA3预处理120小时并在黑暗中培养的种子发芽率为40%。预冷72小时并置于光照下的种子发芽率最高，为60%，置于黑暗中的发芽率为40%。预冷48小时导致种子在光照和黑暗中的发芽率分别为40%和25%。GA3预处理72小时和预冷24小时对诱导种子萌发最有效。以茎尖为外植体，在添加BAP（1 mg/L）+IAA（0.1 mg/L）组合的MS培养基上获得了最大的芽响应。在BAP（1 mg/L）和BAP（1 mg/L）+IAA（0.1 mg/L）上记录到了茎尖的多芽再生。
