Shaver Aaron C, Greig Bruce W, Mosse Claudio A, Seegmiller Adam C
From the Vanderbilt University Medical Center, Nashville, TN, and
From the Vanderbilt University Medical Center, Nashville, TN, and.
Am J Clin Pathol. 2015 May;143(5):716-24. doi: 10.1309/AJCPOOJRAVUN75GD.
Optimizing a clinical flow cytometry panel can be a subjective process dependent on experience. We develop a quantitative method to make this process more rigorous and apply it to B lymphoblastic leukemia/lymphoma (B-ALL) minimal residual disease (MRD) testing.
We retrospectively analyzed our existing three-tube, seven-color B-ALL MRD panel and used our novel method to develop an optimized one-tube, eight-color panel, which was tested prospectively.
The optimized one-tube, eight-color panel resulted in greater efficiency of time and resources with no loss in diagnostic power.
Constructing a flow cytometry panel using a rigorous, objective, quantitative method permits optimization and avoids problems of interdependence and redundancy in a large, multiantigen panel.
优化临床流式细胞术检测方案可能是一个依赖经验的主观过程。我们开发了一种定量方法，以使这一过程更加严谨，并将其应用于B淋巴细胞白血病/淋巴瘤（B-ALL）微小残留病（MRD）检测。
我们回顾性分析了现有的三管、七色B-ALL MRD检测方案，并使用我们的新方法开发了一种优化的单管、八色检测方案，并进行前瞻性测试。
优化后的单管、八色检测方案提高了时间和资源利用效率，且诊断能力没有损失。
使用严谨、客观、定量的方法构建流式细胞术检测方案可实现优化，并避免大型多抗原检测方案中相互依赖和冗余的问题。
