Farris F F, Poklis A, Griesmann G E
J Assoc Off Anal Chem. 1978 May;61(3):660-3.
A method is presented for determining lead in a variety of tissues. Lyophilized samples are solubilized with nitric acid at room temperature in glass screw-cap culture tubes. Following neutralization with sodium hydroxide and sodium bicarbonate, the lead is extracted into methyl isobutyl ketone as the pyrrolidine dithiocarbamate complex and analyzed by flame atomic absorption spectrophotometry. Brain, heart, liver, lung, and spleen gave recoveries ranging from 92 to 102% with standard deviations of less than 8%. Aorta, kidney, and rib were unsuitable for analysis by this method. A large number of samples can be analyzed without specialized equipment or intricate experimental steps. The detection limit is 35 ng/g tissue (wet weight) and sensitivity is approximately 140 ng/g tissue (wet weight).
本文介绍了一种测定多种组织中铅含量的方法。将冻干的样品在室温下于玻璃螺帽培养管中用硝酸溶解。用氢氧化钠和碳酸氢钠中和后，铅以吡咯烷二硫代氨基甲酸盐络合物的形式萃取到甲基异丁基酮中，并通过火焰原子吸收分光光度法进行分析。脑、心脏、肝脏、肺和脾脏的回收率在92%至102%之间，标准偏差小于8%。主动脉、肾脏和肋骨不适合用该方法进行分析。无需专门设备或复杂的实验步骤即可分析大量样品。检测限为35 ng/g组织（湿重），灵敏度约为140 ng/g组织（湿重）。
