Zhang Wen-Jing, Wang Hua, Tong Qiao-Xia, Jie Sheng-Hua, Yang Dong-Liang, Peng Cheng
Department of Infectious Diseases, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, China.
J Huazhong Univ Sci Technolog Med Sci. 2015 Apr;35(2):219-224. doi: 10.1007/s11596-015-1414-5. Epub 2015 Apr 16.
miR-146a is an immunoregulatory microRNA closely associated with viral infection. This study investigated the expression changes of miR-146a in peripheral blood monocytes of HCV-infected patients and the mechanism by which the THP-1 cells were stimulated with HCV core protein in vitro. It was found that in the peripheral blood monocytes of HCV-infected patients, miR-146a expression was upregulated. After treated by interferon/ribavirin, miR-146a expression was decreased when HCV RNA became undetectable. HCV core could directly stimulate THP-1 cells to produce miR-146a. Silencing TLR2 and MyD88 could significantly inhibit the expression of miR-146a. It was concluded that the expression of miR-146a in peripheral blood monocytes of HCV-infected patients was abnormally increased. The TLR2-MyD88 signaling pathway may take part in the overexpression of miR-146a in monocytes stimulated with HCV core protein.
miR-146a是一种与病毒感染密切相关的免疫调节性微小RNA。本研究调查了丙型肝炎病毒（HCV）感染患者外周血单核细胞中miR-146a的表达变化，以及体外使用HCV核心蛋白刺激THP-1细胞的机制。研究发现，在HCV感染患者的外周血单核细胞中，miR-146a表达上调。经干扰素/利巴韦林治疗后，当HCV RNA检测不到时，miR-146a表达下降。HCV核心蛋白可直接刺激THP-1细胞产生miR-146a。沉默TLR2和MyD88可显著抑制miR-146a的表达。得出结论，HCV感染患者外周血单核细胞中miR-146a的表达异常增加。TLR2-MyD88信号通路可能参与了HCV核心蛋白刺激的单核细胞中miR-146a的过表达。
