Gao Wei, Yu Li-Guo, Liu Ya-Li, Wang Yi-Zhao, Huang Xiao-Lin
Department of Traumatic Surgery, Huazhong University of Science and Technology, Wuhan, 430030, China.
Department of Rehabilitation Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China.
J Huazhong Univ Sci Technolog Med Sci. 2015 Apr;35(2):241-247. doi: 10.1007/s11596-015-1418-1. Epub 2015 Apr 16.
The effect of high-frequency repetitive transcranial magnetic stimulation (rTMS) on spasticity following spinal cord injury (SCI) and the action mechanism were investigated. SCI models were established in Sprague-Dawley rats. Five groups were set up: normal control group, SCI-7 day (7D) model group, SCI-14D model group, SCI-7D rTMS group and SCI-14D rTMS group (n=10 each). The rats in SCI rTMS groups were treated with 10 Hz rTMS at 8th day and 15th day after SCI respectively. Motor recovery and spasticity alleviation were evaluated by BBB scale once a week till the end of treatment. Finally, different parts of tissues were dissected out for detection of GABA receptors using Western blotting and polymerase chain reaction (PCR) technique. The results showed that the BBB scores after treatment were significantly higher in SCI-7D rTMS group than in SCI-14D rTMS group (P<0.05). The GABA receptors were down-regulated more significantly in SCI-14D model group than in SCI-7D model group (P<0.05). At different time points, rTMS treatment could affect the up-regulation of GABA receptors: The up-regulation of GABA receptors was more obvious in SCI-7D rTMS group than in SCI-14D rTMS treatment group (P<0.05). It was concluded that 10-Hz rTMS could alleviate spasticity following SCI and promote the motor recovery in rats, which might be attributed to the up-regulation of GABA receptors. It was also suggested that early high-frequency rTMS treatment after SCI may achieve more satisfactory curative effectiveness.
研究了高频重复经颅磁刺激（rTMS）对脊髓损伤（SCI）后痉挛的影响及其作用机制。在Sprague-Dawley大鼠中建立SCI模型。设置五组：正常对照组、SCI-7天（7D）模型组、SCI-14D模型组、SCI-7D rTMS组和SCI-14D rTMS组（每组n = 10）。SCI rTMS组的大鼠分别在SCI后的第8天和第15天接受10 Hz的rTMS治疗。每周用BBB量表评估运动恢复和痉挛缓解情况，直至治疗结束。最后，解剖不同部位的组织，采用蛋白质免疫印迹法和聚合酶链反应（PCR）技术检测GABA受体。结果显示，SCI-7D rTMS组治疗后的BBB评分显著高于SCI-14D rTMS组（P<0.05）。SCI-14D模型组GABA受体的下调比SCI-7D模型组更显著（P<0.05）。在不同时间点，rTMS治疗可影响GABA受体的上调：SCI-7D rTMS组GABA受体的上调比SCI-14D rTMS治疗组更明显（P<0.05）。结论：10 Hz rTMS可减轻SCI后的痉挛并促进大鼠运动恢复，这可能归因于GABA受体的上调。还表明SCI后早期高频rTMS治疗可能取得更满意的疗效。
