Kaminaga K, Noguchi M, Narita A, Sakamoto Y, Kanari Y, Yokoya A
Advanced Science Research Center, Japan Atomic Energy Agency, 2-4 Shirakata-Shirane, Tokai, Ibaraki 319-1195, Japan Graduate School of Science and Engineering, Ibaraki University, 2-1-1 Bunkyo, Mito, Ibaraki 310-8512, Japan.
Advanced Science Research Center, Japan Atomic Energy Agency, 2-4 Shirakata-Shirane, Tokai, Ibaraki 319-1195, Japan.
Radiat Prot Dosimetry. 2015 Sep;166(1-4):91-4. doi: 10.1093/rpd/ncv168. Epub 2015 Apr 15.
To explore the effects of X-ray irradiation on mammalian cell cycle dynamics, single cells using the fluorescent ubiquitination-based cell cycle indicator (Fucci) technique were tracked. HeLa cells expressing Fucci were used to visualise cell cycle modifications induced by irradiation. After cultured HeLa-Fucci cells were exposed to 5 Gy X-rays, fluorescent cell images were captured every 20 min for 48 h using a fluorescent microscope. Time dependence of the fluorescence intensity of S/G2 cells was analysed to examine the cell cycle dynamics of irradiated and non-irradiated control cells. The results showed that irradiated cells could be divided into two populations: one with similar cell cycle dynamics to that of non-irradiated cells, and another displaying a prolonged G2 phase. Based on these findings, it is proposed in this article that an underlying switch mechanism is involved in cell cycle regulation and the G2/M checkpoint of HeLa cells.
为了探究X射线照射对哺乳动物细胞周期动力学的影响，利用基于荧光泛素化的细胞周期指示剂（Fucci）技术对单细胞进行了跟踪。表达Fucci的HeLa细胞用于可视化照射诱导的细胞周期变化。将培养的HeLa-Fucci细胞暴露于5 Gy X射线下后，使用荧光显微镜每20分钟拍摄一次荧光细胞图像，持续48小时。分析S/G2期细胞荧光强度的时间依赖性，以检查照射细胞和未照射对照细胞的细胞周期动力学。结果表明，照射细胞可分为两个群体：一个群体的细胞周期动力学与未照射细胞相似，另一个群体的G2期延长。基于这些发现，本文提出在HeLa细胞的细胞周期调控和G2/M检查点中涉及一种潜在的转换机制。
