用于生物分子可编程释放的DNA链置换反应。

DNA strand displacement reaction for programmable release of biomolecules.

作者信息

Ramezani Hamid, Jed Harrison D

机构信息

Department of Chemistry, University of Alberta, 11227 Saskatchewan Drive NW, Edmonton, AB, T6G 2G2 Canada.

出版信息

Chem Commun (Camb). 2015 May 14;51(39):8307-10. doi: 10.1039/c5cc01880c.

DOI:10.1039/c5cc01880c

PMID:25877680

Abstract

Sample cleanup is a major processing step in many analytical assays. Here, we propose an approach to capture-and-release of analytes based on the DNA strand displacement reaction (SDR) and demonstrate its application to a fluoroimmunoassay on beads for a thyroid cancer biomarker, thyroglobulin. The SDR-based cleanup showed no interference from matrix molecules in serum.

摘要

样品净化是许多分析检测中的一个主要处理步骤。在此，我们提出一种基于DNA链置换反应（SDR）的分析物捕获与释放方法，并展示其在基于珠子的甲状腺癌生物标志物甲状腺球蛋白荧光免疫分析中的应用。基于SDR的净化方法显示不受血清中基质分子的干扰。