Pradhan Nirparaj, Guha Rajdeep, Chowdhury Sushovan, Nandi Sudip, Konar Aditya, Hazra Sarbani
West Bengal University of Animal and Fishery Sciences, 37 & 68 Kshudiram Bose Sarani, Belgachia, Kolkata, 700037, West Bengal, India.
CSIR-Indian Institute of Chemical Biology, 4, Raja SC Mullick Road, Kolkata, 700032, West Bengal, India.
J Mol Med (Berl). 2015 Oct;93(10):1095-106. doi: 10.1007/s00109-015-1277-z. Epub 2015 Apr 17.
Corneal neovascularization is a leading cause for compromised vision. Therapeutic prevention of corneal neovascularization is a major clinical challenge, and there is a compelling need to seek effective and safe therapy for this pathology. This study is aimed to evaluate curcumin nanoparticle for prevention of corneal neovascularization. MePEG-PCL nanoparticles were successfully prepared and characterized. The nanoparticle of curcumin has shown increased efficiency in preventing angiogenic sprouting in vitro. Topical delivery of curcumin nanoparticle in the eye showed enhanced retention of curcumin in the cornea, and significant improvement in prevention of corneal neovascularization over free curcumin as graded clinically and by histopathology; suppression in the expression of VEGF, inflammatory cytokines, and MMP was evidenced in the treated cornea. Curcumin inhibited NFκB in LPS-induced corneal cells. Histopathology and scanning electron microscopy showed absence of any adverse change in the corneal structure following application of curcumin nanoparticle. Therefore, we conclude that curcumin nanoparticle can be a potential candidate for prevention of corneal neovascularization.
• Curcumin nanoparticles show enhanced retention of curcumin in the cornea. • Curcumin NPs suppress the expression of VEGF, inflammatory cytokines, and MMP. • Curcumin NPs prevent corneal neovascularization by suppressing the NFκB pathway. • Curcumin NPs may be a promising candidate for prevention of corneal neovascularization.
角膜新生血管形成是导致视力受损的主要原因。对角膜新生血管形成进行治疗性预防是一项重大临床挑战，迫切需要寻找针对这种病理状况的有效且安全的治疗方法。本研究旨在评估姜黄素纳米颗粒对角膜新生血管形成的预防作用。成功制备并表征了甲氧基聚乙二醇-聚己内酯纳米颗粒。姜黄素纳米颗粒在体外预防血管生成芽生方面显示出更高的效率。在眼部局部递送姜黄素纳米颗粒显示，姜黄素在角膜中的滞留增强，并且在临床分级和组织病理学方面，与游离姜黄素相比，在预防角膜新生血管形成方面有显著改善；在治疗的角膜中证实了血管内皮生长因子（VEGF）、炎性细胞因子和基质金属蛋白酶（MMP）表达受到抑制。姜黄素在脂多糖诱导的角膜细胞中抑制核因子κB（NFκB）。组织病理学和扫描电子显微镜显示，应用姜黄素纳米颗粒后角膜结构未出现任何不良变化。因此，我们得出结论，姜黄素纳米颗粒可能是预防角膜新生血管形成的潜在候选物。
• 姜黄素纳米颗粒在角膜中显示出增强的姜黄素滞留。• 姜黄素纳米颗粒抑制VEGF、炎性细胞因子和MMP的表达。• 姜黄素纳米颗粒通过抑制NFκB途径预防角膜新生血管形成。• 姜黄素纳米颗粒可能是预防角膜新生血管形成的有前途的候选物。
