Lee Sang Gun, Yoo Dae Young, Jung Hyo Young, Nam Sung Min, Kim Jong Whi, Choi Jung Hoon, Yi Sun Shin, Won Moo-Ho, Yoon Yeo Sung, Hwang In Koo, Moon Seung Myung
Departments of Neurosurgery, Dongtan Sacred Heart Hospital, College of Medicine, Hallym University, Hwaseong 445-907, South Korea.
Department of Anatomy and Cell Biology, College of Veterinary Medicine, and Research Institute for Veterinary Science, Seoul National University, Seoul 151-742, South Korea.
Neural Regen Res. 2015 Mar;10(3):451-6. doi: 10.4103/1673-5374.153695.
In this study, we investigated the effects of streptozotocin-induced type 1 diabetes on antioxidant-like protein-1 immunoreactivity, protein carbonyl levels, and malondialdehyde formation, a marker for lipid peroxidation, in the hippocampus. For this study, streptozotocin (75 mg/kg) was intraperitoneally injected into adult rats to induce type 1 diabetes. The three experimental parameters were determined at 2, 3, 4 weeks after streptozotocin treatment. Fasting blood glucose levels significantly increased by 20.7-21.9 mM after streptozotocin treatment. The number of antioxidant-like protein-1 immunoreactive neurons significantly decreased in the hippocampal CA1 region, but not the dentate gyrus, 3 weeks after streptozotocin treatment compared to the control group. Malondialdehyde and protein carbonyl levels, which are modified by oxidative stress, significantly increased with a peak at 3 weeks after malondialdehyde treatment, and then decreased 4 weeks after malondialdehyde treatment. These results suggest that neurons in the hippocampal CA1 region, but not the dentate gyrus, are susceptible to oxidative stress 3 weeks after malondialdehyde treatment.
在本研究中，我们调查了链脲佐菌素诱导的1型糖尿病对海马体中抗氧化样蛋白-1免疫反应性、蛋白质羰基水平以及丙二醛形成（脂质过氧化的标志物）的影响。在本研究中，将链脲佐菌素（75毫克/千克）腹腔注射到成年大鼠体内以诱导1型糖尿病。在链脲佐菌素治疗后的第2、3、4周测定三个实验参数。链脲佐菌素治疗后空腹血糖水平显著升高至20.7 - 21.9毫摩尔。与对照组相比，链脲佐菌素治疗3周后，海马体CA1区中抗氧化样蛋白-1免疫反应性神经元的数量显著减少，但齿状回中未减少。经氧化应激修饰的丙二醛和蛋白质羰基水平在链脲佐菌素治疗3周时显著升高并达到峰值，然后在治疗4周后下降。这些结果表明，链脲佐菌素治疗3周后，海马体CA1区的神经元而非齿状回的神经元易受氧化应激影响。
