National Engineering Laboratory for Modern Silk, College of Textile and Clothing Engineering, Soochow University, Suzhou, 215123, P. R. China.
Department of Biomedical Engineering, Tufts University, 4 Colby St., Room 153, Medford, MA, 02155, USA.
Macromol Biosci. 2015 Aug;15(8):1125-33. doi: 10.1002/mabi.201400502. Epub 2015 Apr 16.
The fabrication of structural mimetic scaffolds reinforced with multi-angle silk fibers is described. Degummed silk fibers with a parallel arrangement of the fibers in a planar format were overlapped with successive layers organized at 0°, 30°, 60° and 90°, respectively. The overlapped silk fiber layers were coated with silk solution (6 wt%) containing sodium dodecyl sulfate (SDS). The morphology, mechanical properties, structure and biocompatibility of the scaffolds were investigated. The mechanical properties of the scaffolds (tensile and burst) were characterized based on the angles of the fibers. Layers with an overlapping angle at 30° exhibited better mechanical performance (18 MPa) than the other groups. The results of Fourier Transform (FT) IR Spectroscopy (FT-IR) and X-ray Differentiation (XRD) analyses indicated that the presence of degummed silk fibers with different angles did not significantly impact secondary structure or crystallization of the fiber reinforced scaffolds. The attachment and growth of a human fibroblast cell line (HS-865-SK) on the reinforced scaffolds supported good cell compatibility. These new scaffolds have potential applications in tissue repairs where superior mechanical strength and cell compatibility are important.
本文描述了具有多向丝纤维增强的结构模拟支架的制造。将纤维平行排列的脱胶丝纤维以重叠的方式，分别以 0°、30°、60°和 90°的角度进行层叠。重叠的丝纤维层用含有十二烷基硫酸钠（SDS）的丝溶液（6wt%）进行涂层。对支架的形态、力学性能、结构和生物相容性进行了研究。基于纤维的角度，对支架的力学性能（拉伸和爆裂）进行了表征。具有 30°重叠角的层表现出比其他组更好的力学性能（18MPa）。傅里叶变换（FT）红外光谱（FT-IR）和 X 射线衍射（XRD）分析结果表明，不同角度的脱胶丝纤维的存在对纤维增强支架的二级结构或结晶没有显著影响。在增强支架上附着和生长的人成纤维细胞系（HS-865-SK）支持良好的细胞相容性。这些新型支架在需要优异的机械强度和细胞相容性的组织修复中有潜在的应用。
