巨大芽孢杆菌孢子和细胞中的烯醇化酶：该酶的两步纯化及其某些性质
Enolase from spores and cells of Bacillus megaterium: two-step purification of the enzyme and some of its properties.
作者信息
Singh R P, Setlow P
出版信息
J Bacteriol. 1978 Apr;134(1):353-5. doi: 10.1128/jb.134.1.353-355.1978.
Abstract
A simple two-step procedure for purification of enolase from germinated spores or vegetative cells of Bacillus megaterium is described. The procedure resulted in a 1,200-fold purification with production of homogeneous enzyme in approximately 75% yield; the enzymes from spores and cells seemed identical. The molecular weight of the native enzyme was 335,000, with a subunit molecular weight of 42,000. The enzyme required Mg2+ and was inhibited by ethylenediaminetetraacetic acid and fluoride ions. The Michaelis constants for 2-phosphoglyceric acid and Mg2+ were 7.1 X 10(-4) and 4.7 X 10(-4) M, respectively.
摘要
本文描述了一种从巨大芽孢杆菌的萌发孢子或营养细胞中纯化烯醇化酶的简单两步法。该方法实现了1200倍的纯化，产生的均一酶产量约为75%；来自孢子和细胞的酶似乎相同。天然酶的分子量为335,000，亚基分子量为42,000。该酶需要Mg2+，并受到乙二胺四乙酸和氟离子的抑制。2-磷酸甘油酸和Mg2+的米氏常数分别为7.1×10(-4)和4.7×10(-4)M。
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本文引用的文献
1
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
2
A PURIFICATION OF BREWERS' AND BAKERS' YEAST ENOLASE YIELDING A SINGLE ACTIVE COMPONENT.啤酒酵母和面包酵母烯醇化酶的纯化，得到单一活性成分。
J Biol Chem. 1964 Aug;239:2464-8.
3
The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis.通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳测定分子量的可靠性。
J Biol Chem. 1969 Aug 25;244(16):4406-12.
4
Biochemical studies of bacterial sporulation and germination. XIX. Phosphate metabolism during sporulation.细菌孢子形成与萌发的生化研究。第十九部分。孢子形成过程中的磷酸盐代谢。
J Biol Chem. 1970 Mar 10;245(5):1137-45.
5
The purification and characterization of Escherichia coli enolase.大肠杆菌烯醇化酶的纯化与特性分析。
J Biol Chem. 1971 Nov 25;246(22):6797-802.
6
A thermostable enolase from the extreme thermophile Thermus aquaticus YT-1.
Biochemistry. 1973 Apr 10;12(8):1552-9. doi: 10.1021/bi00732a014.
7
Enolase from the thermophile Thermus X-1.
Biochemistry. 1973 Apr 10;12(8):1559-65. doi: 10.1021/bi00732a015.
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9
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