Otani M, Ihara N, Umezawa C, Sano K
J Bacteriol. 1986 Jul;167(1):148-52. doi: 10.1128/jb.167.1.148-152.1986.
Metabolic pathways of glucose during germination of Bacillus megaterium QM B1551 spores were studied by using specifically labeled glucose and gluconate. The Embden-Meyerhof pathway, the pentose cycle, and the direct oxidation route of glucose to gluconate (the gluconate pathway) were all operative at this stage; among those, gluconate accumulation was most predominant, especially in the early stage. Potassium fluoride, an enolase inhibitor, abolished the catabolism by the Embden-Meyerhof pathway totally without affecting gluconate accumulation. Under these conditions glucose was exclusively oxidized to gluconate. Gluconate thus accumulated could be metabolized further via phosphorylation by gluconate kinase. Remarkable gluconate accumulation was also demonstrated in several other spores requiring alanine as an effective germinant. NADH formed by the direct glucose oxidation may serve as a initial ATP source to phosphorylate glucose in germinating spores.
通过使用特异性标记的葡萄糖和葡萄糖酸盐，研究了巨大芽孢杆菌QM B1551孢子萌发过程中葡萄糖的代谢途径。糖酵解途径、戊糖循环以及葡萄糖直接氧化为葡萄糖酸盐的途径（葡萄糖酸盐途径）在这个阶段都发挥作用；其中，葡萄糖酸盐的积累最为显著，尤其是在早期。烯醇化酶抑制剂氟化钾完全消除了糖酵解途径的分解代谢，而不影响葡萄糖酸盐的积累。在这些条件下，葡萄糖仅被氧化为葡萄糖酸盐。如此积累的葡萄糖酸盐可通过葡萄糖酸盐激酶磷酸化进一步代谢。在其他几种需要丙氨酸作为有效萌发剂的孢子中也观察到显著的葡萄糖酸盐积累。由葡萄糖直接氧化形成的NADH可能作为初始ATP来源，用于使萌发孢子中的葡萄糖磷酸化。
