检测IgG4相关疾病及其他病症患者的血清IgG4水平。
Detection of serum IgG4 levels in patients with IgG4-related disease and other disorders.
作者信息
Su Yuying, Sun Wei, Wang Chenqiong, Wu Xuefen, Miao Ye, Xiong Hui, Bai Lin, Dong Lingli
机构信息
Department of Rheumatology and Immunology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, China.
Department of Stomatology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, China.
出版信息
PLoS One. 2015 Apr 17;10(4):e0124233. doi: 10.1371/journal.pone.0124233. eCollection 2015.
OBJECTIVE
Elevated serum IgG4 levels are an important hallmark for diagnosing IgG4-related disease (IgG4-RD), but can also be observed in other diseases. This study aimed to compare two different testing methods for IgG4: ELISA and nephelometric assay. Both assays were used to measure serum IgG4 concentrations, and to assess the prevalence of high serum IgG4 levels in both IgG4-RD and non-IgG4-RD diseases.
METHODS
A total of 80 serum samples were tested using the nephelometric assay and ELISA method that we established. Serum IgG4 concentrations were determined by ELISA for 957 patients with distinct diseases, including 12 cases of IgG4-RD and 945 cases of non-IgG4-RD.
RESULTS
IgG4 levels from 80 selected serum samples examined by ELISA were in agreement with those detected using the nephelometry assay. Meanwhile, the serum IgG4 concentrations measured by ELISA were also consistent with the clinical diagnoses of patients with IgG4-RD during the course of disease. The Elevated levels of serum IgG4 (>1.35 g/L) were detected in all IgG4-RD (12/12) patients, and the prevalence of high IgG4 serum levels was 3.39% in non-IgG4-RD cases. Among them, the positive rates of serum IgG4 were 2.06% in patients with carcinoma and 6.3% in patients with other non-IgG4 autoimmune diseases.
CONCLUSION
Our established ELISA method is a reliable and convenient technique, which could be extensively used in the clinic to measure serum IgG4 levels. High levels of IgG4 were observed in IgG4-RD. However, this phenomenon could also be observed in other diseases, such as carcinomas and other autoimmune diseases. Thus, a diagnosis of IgG4 disease cannot only be dependent on the detection of elevated serum IgG4 levels.
目的
血清IgG4水平升高是诊断IgG4相关疾病（IgG4-RD）的重要标志，但在其他疾病中也可观察到。本研究旨在比较两种不同的IgG4检测方法：酶联免疫吸附测定（ELISA）和散射比浊法。两种检测方法均用于测量血清IgG4浓度，并评估IgG4-RD和非IgG4-RD疾病中高血清IgG4水平的患病率。
方法
使用我们建立的散射比浊法和ELISA方法对总共80份血清样本进行检测。通过ELISA测定了957例患有不同疾病患者的血清IgG4浓度，其中包括12例IgG4-RD患者和945例非IgG4-RD患者。
结果
ELISA检测的80份选定血清样本中的IgG4水平与散射比浊法检测的结果一致。同时，ELISA测量的血清IgG4浓度也与IgG4-RD患者病程中的临床诊断一致。所有IgG4-RD（12/12）患者均检测出血清IgG4水平升高（>1.35 g/L），非IgG4-RD病例中高IgG4血清水平的患病率为3.39%。其中，癌症患者血清IgG4阳性率为2.06%，其他非IgG4自身免疫性疾病患者为6.3%。
结论
我们建立的ELISA方法是一种可靠且便捷的技术，可在临床上广泛用于测量血清IgG4水平。在IgG4-RD中观察到IgG较水平升高。然而，在其他疾病中也可观察到这种现象，如癌症和其他自身免疫性疾病。因此，IgG4疾病的诊断不能仅依赖于血清IgG4水平升高的检测。
Zotero 插件