School of Chemical Engineering, Universiti Sains Malaysia, Engineering Campus, 14300 Nibong Tebal, Penang, Malaysia.
School of Chemical Engineering, Universiti Sains Malaysia, Engineering Campus, 14300 Nibong Tebal, Penang, Malaysia.
Bioresour Technol. 2015;189:169-176. doi: 10.1016/j.biortech.2015.04.023. Epub 2015 Apr 11.
Polyhydroxyalkanoate (PHA) recovery from aerobic granules was investigated using four cell digestion agents, namely, sodium hypochlorite, sodium hydroxide, acetone and sodium chloride. Simultaneously, the removal of extracellular polymeric substances (EPS) and its effect on PHA yield were investigated. The highest PHA recovery yield was obtained using sodium hypochlorite, accounting for 89% cell dry weight (CDW). The highest PHA was recovered after the sodium hypochlorite completely removed the EPS from the aerobic granules. The average molecular weight (Mw) of the PHA recovered using sodium hypochlorite was 5.31 × 10(5)g/mol with only 1.8% molecular weight degradation. The energy and duration analysis for PHA recovery revealed that the sodium hypochlorite method required the least amount of energy and time at 0.0561 MJ/g PHA and 26 h, respectively. The PHA that was recovered was a P3(HB-co-HV) co-polymer.
聚羟基烷酸酯（PHA）从好氧颗粒中回收利用，使用了四种细胞消化剂，即次氯酸钠、氢氧化钠、丙酮和氯化钠。同时，还研究了细胞外聚合物（EPS）的去除及其对 PHA 产率的影响。使用次氯酸钠获得了最高的 PHA 回收产率，占细胞干重（CDW）的 89%。当次氯酸钠完全从好氧颗粒中去除 EPS 时，可回收最多的 PHA。使用次氯酸钠回收的 PHA 的平均分子量（Mw）为 5.31×10（5）g/mol，分子量降解率仅为 1.8%。PHA 回收的能量和持续时间分析表明，次氯酸钠法所需的能量和时间最少，分别为 0.0561 MJ/g PHA 和 26 h。回收的 PHA 是一种 P3（HB-co-HV）共聚物。
