WPI-Advanced Institute for Materials Research, Tohoku University, Sendai 980-8578, Japan; Department of Life Science and Medical Bioscience, Graduate School of Advanced Science and Engineering, Waseda University, Tokyo, Japan.
WPI-Advanced Institute for Materials Research, Tohoku University, Sendai 980-8578, Japan.
Biomaterials. 2015 Jun;53:86-94. doi: 10.1016/j.biomaterials.2015.02.028. Epub 2015 Mar 9.
Spatial coordination of cell orientation is of central importance in tissue/organ construction. In this study, we developed microfabricated poly(lactic-co-glycolic acid) (PLGA) nanoribbon sheets with unique structures, using spin-coating and micropatterning techniques, in order to generate a hierarchically assembled cellular structure consisting of murine skeletal myoblasts (C2C12). The nanoribbon sheets were composed of aligned PLGA nanoribbons in the center, and strips on four sides which take a role as bridges to connect and immobilize the aligned nanoribbons. Such unique structures facilitated the alignment of C2C12 cells into bilayer cell sheets, and cellular alignment was directed by the aligned direction of nanoribbons. The nanoribbon sheets also facilitated the construction of multilayer cell sheets with anisotropic (orthogonal) and isotropic (parallel) orientations. The enhanced expression of myogenic genes of C2C12 cells on the bilayer cell sheets demonstrated that the nanoribbons induced C2C12 cell differentiation into mature myoblasts. The micropatterned nanoribbon sheets may be a useful tool for directing cellular organization with defined alignment for regenerative medicine and drug screening applications.
细胞方向的空间协调在组织/器官构建中具有核心重要性。在这项研究中，我们使用旋涂和微图案化技术开发了具有独特结构的微制造聚（乳酸-共-乙醇酸）（PLGA）纳米带片，以生成由鼠骨骼肌成肌细胞（C2C12）组成的层次组装细胞结构。纳米带片由中心的对齐 PLGA 纳米带和四条边上的条带组成，这些条带作为桥梁连接和固定对齐的纳米带。这种独特的结构促进了 C2C12 细胞排列成双层细胞片，并且细胞排列由纳米带的对齐方向引导。纳米带片还促进了具有各向异性（正交）和各向同性（平行）取向的多层细胞片的构建。C2C12 细胞在双层细胞片上的肌生成基因表达增强表明，纳米带诱导 C2C12 细胞分化为成熟成肌细胞。这种微图案化的纳米带片可能是用于引导具有定义对齐的细胞组织的有用工具，可用于再生医学和药物筛选应用。
