Fazekas S, Ováry I, Székessy-Hermann V, Magyar I
Second Institute of Biochemistry, Semmeiweis University Medical School, Budapest, Hungary.
Acta Physiol Hung. 1989;73(4):403-16.
Myosin concentrations and their endogenous phosphate (P) content as well as some of their properties were investigated in different parts of the central nervous system (CNS). Myosin concentration in hemispherial part is in the average 40 mg/100 g fresh mince, but it varies between 5 mg and 30 mg myosin depending on the mental activity or accidental brain diseases. This refers to the other parts of CNS, too. A myosin may be isolated with a single and sharp peak as far as the gel filtration profile is concerned in spite of the high lipid content of the brain. Working with minimal lipid and RNA content requires a careful procedure by an experienced person. In spite of any careful isolation procedure, purified KCl-myosin preparations are composed of at least two isomyosins each formed from different heavy chains. Myosins are localized in different parts of the cell, mitochondrial, synaptosomal and neuroplasmic, respectively. Brain myosins have higher endogeneous P concentrations than other cell-myosins. In hemispherial myosins the P-concentrations are high. Subjects trained in mental activities have the highest myosin and P concentration. We suggest that the role of myosin may directly be connected with cytokinetic motions even in case of the higher functions of brain cells like thinking, paying attention and memory, respectively.
研究了中枢神经系统（CNS）不同部位的肌球蛋白浓度及其内源性磷酸盐（P）含量以及它们的一些特性。半球部分的肌球蛋白浓度平均为40mg/100g新鲜碎肉，但根据精神活动或意外脑部疾病的不同，其肌球蛋白含量在5mg至30mg之间变化。这也适用于中枢神经系统的其他部位。尽管大脑脂质含量很高，但就凝胶过滤图谱而言，肌球蛋白可以以单一且尖锐的峰被分离出来。处理脂质和RNA含量极低的样本需要经验丰富的人员进行仔细操作。尽管采用了任何仔细的分离程序，纯化的KCl-肌球蛋白制剂至少由两种同功肌球蛋白组成，每种同功肌球蛋白由不同的重链形成。肌球蛋白分别定位于细胞的不同部位，即线粒体、突触体和神经浆中。脑肌球蛋白的内源性P浓度高于其他细胞肌球蛋白。在半球肌球蛋白中，P浓度很高。接受过精神活动训练的受试者肌球蛋白和P浓度最高。我们认为，即使在脑细胞执行诸如思考、注意力集中和记忆等高级功能的情况下，肌球蛋白的作用也可能直接与细胞动力学运动相关。
