姜黄素微复合物负载壳聚糖支架的体外生物相容性及姜黄素释放
In vitro biocompatibility and release of curcumin from curcumin microcomplex-loaded chitosan scaffold.
作者信息
Amirthalingam Muthukumar, Kasinathan Narayanan, Mutalik Srinivas, Udupa Nayanabhirama
机构信息
Department of Pharmaceutics, Manipal College of Pharmaceutical Sciences, Manipal University , Manipal , India .
出版信息
J Microencapsul. 2015;32(4):364-71. doi: 10.3109/02652048.2015.1028496. Epub 2015 Apr 20.
CONTEXT
Scaffold if suitably modified could be used as a drug delivery system.
OBJECTIVE
To develop chitosan scaffold as a delivery system for delivering curcumin in wound-healing application.
MATERIALS AND METHODS
Chitosan-curcumin microcomplex particles were prepared, and the effect of drug-polymer ratio (DPR) and homogenisation speed (HS) was studied using a two-level full-factorial design. Chitosan scaffold was prepared and incorporated with curcumin microcomplexes to obtain a chitosan scaffold-containing chitosan-curcumin microcomplex (CS-CCM). Antimicrobial property of the CS-CCM against Escherichia coli was studied. The cytotoxicity of CS-CCM was studied by assessing the cell viability by MTT assay.
RESULTS AND DISCUSSION
DPR had a significant effect (p ≤ 0.05) on the drug content. CS-CCM was able to inhibit the growth of E. coli considerably. The MTT results showed that CS-CCM is non-cytotoxic and supports cell proliferation.
CONCLUSION
CS-CCM due to its biocompatibility and antimicrobial property could be further evaluated for potential application in wound healing.
背景
如果进行适当修饰，支架可作为药物递送系统。
目的
开发壳聚糖支架作为一种在伤口愈合应用中递送姜黄素的递送系统。
材料与方法
制备壳聚糖 - 姜黄素微复合物颗粒，并采用二级全因子设计研究药物 - 聚合物比例（DPR）和均质速度（HS）的影响。制备壳聚糖支架并与姜黄素微复合物结合，以获得含壳聚糖 - 姜黄素微复合物的壳聚糖支架（CS - CCM）。研究了CS - CCM对大肠杆菌的抗菌性能。通过MTT法评估细胞活力来研究CS - CCM的细胞毒性。
结果与讨论
DPR对药物含量有显著影响（p≤0.05）。CS - CCM能够显著抑制大肠杆菌的生长。MTT结果表明CS - CCM无细胞毒性并支持细胞增殖。
结论
由于其生物相容性和抗菌性能，CS - CCM可进一步评估其在伤口愈合中的潜在应用。
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