Kaviani Maryam, Geramizadeh Bita, Rahsaz Marjan, Marzban Saeed
From the Shiraz Transplant Research Center, Shiraz, Iran.
Exp Clin Transplant. 2015 Apr;13 Suppl 1:366-70.
Large-scale expansion of epidermal keratinocytes is essential in the application of these cells for severe burn treatment in patients. Therefore, this study was designed to evaluate various conditions in the expansion of human epidermal keratinocytes.
The epidermis was separated from the dermis of skin samples using dispase. The epidermis was trypsinized for keratinocyte isolation. Keratinocytes were cultured in various conditions, with or without a human dermal fibroblast feeder layer, mitomycin C treatment, and different culture media.
Our results suggest that keratinocytes cultured on a human dermal fibroblast feeder layer were grown for several passages. Extensive deformation and rapid deterioration were observed in the cultured cells without a feeder layer and in serum-free medium.
Human dermal fibroblasts treated with mitomycin C can provide optimal conditions for proliferation of keratinocytes.
在将这些细胞应用于严重烧伤患者的治疗中，大规模扩增表皮角质形成细胞至关重要。因此，本研究旨在评估人表皮角质形成细胞扩增的各种条件。
使用Dispase从皮肤样本的真皮中分离表皮。将表皮用胰蛋白酶处理以分离角质形成细胞。角质形成细胞在各种条件下培养，有或没有人类真皮成纤维细胞饲养层、丝裂霉素C处理以及不同的培养基。
我们的结果表明，在人类真皮成纤维细胞饲养层上培养的角质形成细胞能够传代培养数代。在没有饲养层的培养细胞和无血清培养基中观察到广泛的变形和快速退化。
用丝裂霉素C处理的人类真皮成纤维细胞可为角质形成细胞的增殖提供最佳条件。
