Červená Kateřina, Pačesová Dominika, Spišská Veronika, Bendová Zdeňka
Department of Physiology, Faculty of Science, Charles University in Prague, Viničná 7, Prague 2, 128 43, Czech Republic.
J Mol Neurosci. 2015 Jun;56(2):371-6. doi: 10.1007/s12031-015-0563-0. Epub 2015 Apr 17.
The intrinsic period of circadian clock in the suprachiasmatic nucleus is entrained to a 24-h cycle by external cues, mainly light. Previous studies have shown that light applied at night induces robust phosphorylation of extracellular-signal-regulated kinase that is necessary to process the light pulse into the phase shift of the clock phase. In this study, we show the persistent downregulation of phosphorylated extracellular-signal-regulated kinase and transient downregulation of phosphorylated glycogen synthase kinase-3beta in the ventrolateral part of the suprachiasmatic nucleus to photic stimuli starting at 2 h after the beginning of the light pulse. As both kinases are involved in regulation of circadian clockwork, we hypothesize that these changes may contribute to the phase-shifting effect of light at night.
视交叉上核生物钟的内在周期通过外部线索（主要是光线）被调整为24小时周期。先前的研究表明，夜间施加的光线会诱导细胞外信号调节激酶的强烈磷酸化，这是将光脉冲转化为生物钟相位偏移所必需的。在本研究中，我们发现，从光脉冲开始后2小时起，对视交叉上核腹外侧部分的光刺激会导致磷酸化细胞外信号调节激酶持续下调，以及磷酸化糖原合酶激酶-3β短暂下调。由于这两种激酶都参与生物钟机制的调节，我们推测这些变化可能有助于夜间光线的相位偏移效应。
