Verma Dev Kumar, Rathore Gaurav, Pradhan Pravata Kumar, Sood Neeraj, Punia Peyush
J Environ Biol. 2015 Mar;36(2):433-9.
Filamentous bacteria overlaying ulcerated area on the body surface were observed in the wet-mout preparation from a moribund goldfish with saddle back appearance. The causative agent was identified as Flavobacterium columnrae, on the basis of biochemical test, species-specific polymerase chain reaction (PCR) and sequencing of 16S rDNA gene with the universal bacterial primers. Furthermore, the strain (ING-1) attributed to genomovar II in 16S rDNA PCR-restriction fragment length polymorphism (PCR-RFLP) and sequence analysis. In phylogenetic analysis, the strain ING-1, produced typical columnaris disease symptoms in rohu (Labeo rohita) fingerlings within 10 days. This is a new record about molecular detection and identification of Flavobacterium columnare, occurring naturally on a new host Carassius auratusin India.
在一条呈现鞍背外观的濒死金鱼的湿片标本中，观察到体表溃疡区域覆盖有丝状细菌。基于生化试验、种特异性聚合酶链反应（PCR）以及使用通用细菌引物对16S rDNA基因进行测序，确定病原体为柱状黄杆菌。此外，该菌株（ING-1）在16S rDNA PCR-限制性片段长度多态性（PCR-RFLP）和序列分析中属于基因变种II。在系统发育分析中，ING-1菌株在10天内使露斯塔野鲮（Labeo rohita）幼鱼出现典型的柱状病症状。这是关于柱状黄杆菌在印度新宿主金鱼上自然发生的分子检测和鉴定的新记录。
