Zhang Chao-qiang, Wang Xing-long, Yang Zhi-yuan, Zheng Xiu-fang
Zhong Yao Cai. 2014 Oct;37(10):1725-9.
To investigate the effect of NaCl stress on the growth and physiological characteristics of Physalis alkekengi var. franchetii, and to discuss the mechanism of salt tolerance.
The test-tube plantlet was cultured on the stress medium with NaCl (0.2%~1.0%), the content of proline, soluble sugar, soluble protein, chlorophyll and malondialdthyde (MDA), and membrane relative permeability, as well as the activities of superoxide dismutase (SOD), catalase (CAT) and peroxidase (POD) were determined and analyzed.
The average height, stem diameter, root length, number of root and chlorophyll content gradually decreased at salinity less than or equal 0.6% NaCl, then decreased rapidly at salinity greater than or equal 0.8% NaCl, while the root formation was significantly inhibited. The content of free proline and SOD activities always increased with NaCl concentration increasing (0.2%~ 1.0%). Nevertheless, the contents of soluble sugar, soluble protein and the activities of CAT and POD increased firstly and then decreased, but the contents of soluble sugar and soluble protein were higher than those of the control group, whereas the content of MDA and membrane relative permeability accumulated slowly at salinity less than or equal 0.6% NaCl, then increased rapidly at salinity greater than 0.8% NaCl.
The test-tube plantlet has an adaptability to salty environment at salinity less than or equal 0.6% NaCl, and the test-tube plantlet can alleviate the salty injury by increasing the contents of osmotic regulation substances, such as soluble sugar and soluble protein, decreasing the osmotic potential, and increasing the activities of antioxidant enzymes.
研究NaCl胁迫对酸浆生长及生理特性的影响，探讨其耐盐机制。
将试管苗培养在含NaCl（0.2%~1.0%）的胁迫培养基上，测定并分析脯氨酸、可溶性糖、可溶性蛋白、叶绿素和丙二醛（MDA）含量、膜相对透性以及超氧化物歧化酶（SOD）、过氧化氢酶（CAT）和过氧化物酶（POD）活性。
在NaCl浓度小于或等于0.6%时，试管苗平均株高、茎粗、根长、根数及叶绿素含量逐渐降低，在NaCl浓度大于或等于0.8%时则迅速下降，且生根受到显著抑制。游离脯氨酸含量和SOD活性随NaCl浓度升高（0.2%~1.0%）而始终增加。然而，可溶性糖、可溶性蛋白含量以及CAT和POD活性先升高后降低，但可溶性糖和可溶性蛋白含量高于对照组，在NaCl浓度小于或等于0.6%时MDA含量和膜相对透性积累缓慢，在NaCl浓度大于0.8%时迅速增加。
试管苗在NaCl浓度小于或等于0.6%时对盐环境具有一定适应性，可通过增加可溶性糖和可溶性蛋白等渗透调节物质含量、降低渗透势以及提高抗氧化酶活性来缓解盐害。
