Gao Li, Li Chao, Yang Ran-Yao, Lian Wen-Wen, Fang Jian-Song, Pang Xiao-Cong, Qin Xue-Mei, Liu Ai-Lin, Du Guan-Hua
Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, PR China; Modern Research Center for Traditional Chinese Medicine of Shanxi University, Taiyuan 030006, PR China.
Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, PR China.
Pharmacol Biochem Behav. 2015 Jun;133:155-63. doi: 10.1016/j.pbb.2015.04.004. Epub 2015 Apr 18.
Baicalein, a flavonoid from Scutellaria baicalensis Georgi, has been shown to possess neuroprotective properties. The purpose of this study was to explore the effects of baicalein on motor behavioral deficits and gene expression in N-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-induced mice model of Parkinson's disease (PD). The behavioral results showed that baicalein significantly improves the abnormal behaviors in MPTP-induced mice model of PD, as manifested by shortening the total time for climbing down the pole, prolonging the latent periods of rotarod, and increasing the vertical movements. Using cDNA microarray and subsequent bioinformatic analyses, it was found that baicalein significantly promotes the biological processes including neurogenesis, neuroblast proliferation, neurotrophin signaling pathway, walking and locomotor behaviors, and inhibits dopamine metabolic process through regulation of gene expressions. Based on analysis of gene co-expression networks, the results indicated that the regulation of genes such as LIMK1, SNCA and GLRA1 by baicalein might play central roles in the network. Our results provide experimental evidence for the potential use of baicalein in the treatment of PD, and revealed gene expression profiles, biological processes and pathways influenced by baicalein in MPTP-treated mice.
黄芩素是从黄芩中提取的一种黄酮类化合物，已被证明具有神经保护特性。本研究的目的是探讨黄芩素对1-甲基-4-苯基-1,2,3,6-四氢吡啶（MPTP）诱导的帕金森病（PD）小鼠模型运动行为缺陷和基因表达的影响。行为学结果表明，黄芩素显著改善了MPTP诱导的PD小鼠模型中的异常行为，表现为缩短爬杆总时间、延长转棒试验潜伏期以及增加垂直运动。通过cDNA微阵列和后续生物信息学分析发现，黄芩素通过调节基因表达显著促进包括神经发生、神经母细胞增殖、神经营养因子信号通路、行走和运动行为等生物学过程，并抑制多巴胺代谢过程。基于基因共表达网络分析，结果表明黄芩素对LIMK1、SNCA和GLRA1等基因的调控可能在该网络中起核心作用。我们的研究结果为黄芩素在PD治疗中的潜在应用提供了实验证据，并揭示了黄芩素在MPTP处理小鼠中影响的基因表达谱、生物学过程和信号通路。
