Vaughan Robert C, Kao C Cheng
Department of Molecular and Cellular Biochemistry, Indiana University, 212 S. Hawthorne St., 201A Simon Hall, Bloomington, IN, 47405, USA,
Methods Mol Biol. 2015;1297:225-36. doi: 10.1007/978-1-4939-2562-9_16.
RNA nanotechnology often feature protein RNA complexes. The interaction between proteins and large RNAs are difficult to study using traditional structure-based methods like NMR or X-ray crystallography. RCAP, an approach that uses reversible-cross-linking affinity purification method coupled with mass spectrometry, has been developed to map regions within proteins that contact RNA. This chapter details how RCAP is applied to map protein-RNA contacts within virions.
RNA纳米技术常常以蛋白质-RNA复合物为特征。使用诸如核磁共振（NMR）或X射线晶体学等传统的基于结构的方法，很难研究蛋白质与大型RNA之间的相互作用。已经开发出一种名为RCAP的方法，即结合质谱的可逆交联亲和纯化方法，用于绘制蛋白质中与RNA接触的区域。本章详细介绍了如何应用RCAP来绘制病毒粒子内的蛋白质-RNA接触位点。
