Wang Lih-Chiann, Huang Dean, Cheng Ming-Chu, Lee Shu-Hwae, Wang Ching-Ho
School of Veterinary Medicine, National Taiwan University, No. 1, Sec. 4, Roosevelt Road, Taipei 10617, Taiwan.
Animal Health Research Institute, Council of Agriculture, Taipei, Taiwan.
J Virol Methods. 2015 Aug;220:39-42. doi: 10.1016/j.jviromet.2015.04.006. Epub 2015 Apr 17.
The H5 avian influenza virus subtype has huge impact on the poultry industry. Rapid diagnosis and accurate identification of the highly pathogenic avian influenza virus and low-pathogenicity avian influenza virus is essential, especially during H5 outbreaks and surveillance. To this end, a novel and rapid strategy for H5 virus molecular pathotyping is presented. The specific hemagglutinin gene of the H5 virus and the basic amino acid number of the motif at the hemagglutinin precursor protein cleavage site were detected using oligonucleotide microarray. Highly pathogenic and low-pathogenicity avian influenza viruses in Taiwan were differentiated using 13 microarray probes with the naked eye. The detection limit reached 3.4 viral RNA copies, 1000 times more sensitive than reverse transcription polymerase chain reaction. Thus, the oligonucleotide microarray would provide an alternative H5 pathogenicity determination using the naked eye for laboratories lacking facilities.
H5亚型禽流感病毒对家禽业有巨大影响。快速诊断并准确鉴定高致病性禽流感病毒和低致病性禽流感病毒至关重要，尤其是在H5疫情爆发及监测期间。为此，提出了一种新型快速的H5病毒分子分型策略。使用寡核苷酸微阵列检测H5病毒的特异性血凝素基因以及血凝素前体蛋白裂解位点基序的碱性氨基酸数量。用13个微阵列探针肉眼区分台湾的高致病性和低致病性禽流感病毒。检测限达到3.4个病毒RNA拷贝，比逆转录聚合酶链反应灵敏1000倍。因此，对于缺乏相关设备的实验室，寡核苷酸微阵列可为肉眼判定H5致病性提供一种替代方法。
