Kumar Rishikesh, Rafia Sheikh, Saran Shweta
School of Life Sciences, Jawaharlal Nehru University, New Delhi, India.
Int J Dev Biol. 2014;58(9):669-76. doi: 10.1387/ijdb.140174ss.
Ornithine decarboxylase (ODC) is a rate limiting enzyme in polyamine synthesis that decarboxylates ornithine to form the diamine putrescine. We report here the isolation, expression and characterization of a homolog of ODC from Dictyostelium discoideum. DdODC is conserved and shows sequence and structural homology with that from human. Both ODC transcript and protein are expressed at all stages of development and show high expression in prestalk/stalk cells. It is cytosolic and predominantly perinuclear in localization. Both overexpression of DdODC and putrescine treatment resulted in inhibition of cell proliferation.
鸟氨酸脱羧酶（ODC）是多胺合成中的一种限速酶，它使鸟氨酸脱羧形成二胺腐胺。我们在此报告从盘基网柄菌中分离、表达并鉴定ODC的一个同源物。盘基网柄菌ODC（DdODC）具有保守性，与人类ODC在序列和结构上具有同源性。ODC转录本和蛋白质在发育的各个阶段均有表达，且在前柄/柄细胞中高表达。它位于细胞质中，主要定位于核周。DdODC的过表达和腐胺处理均导致细胞增殖受到抑制。
